松针粉对柴鸡血清免疫球蛋白、生殖激素和肠道菌群的影响  

Kffofts of pi no needlo powder on serum immunoglobulin, reproductive hormone and intestinal flora of free-range layers

作　　者：王净[1] 李寸欣[1] 王鹏[2] 王理超[1] 马晋[1] 张石磊[1] 

机构地区：[1]河北北方学院动物科技学院,河北张家口075131 [2]河北北方学院农林科技学院,河北张家口075131

出　　处：《中国饲料》2015年第7期21-23,共3页China Feed

基　　金：河北省科技计划项目[11230405D-1(2013)-4、14236602D-(2014)];河北省畜牧兽医局科技项目(2012-3-03)

摘　　要：本研究在柴鸡饲粮中添加不同比例的松针粉,探讨其对柴鸡血清免疫球蛋白、生殖激素及肠道菌群的影响。试验选取120只160日龄、体重约1.0 kg的健康柴鸡作为供试鸡,分为4个处理,每个处理3个重复,每个重复10只鸡。在基础日粮中分别添加0%、3%、5%、7%的松针粉。结果表明：与对照组相比,试验Ⅰ、Ⅱ、Ⅲ组Ig A含量分别提高23.12%、18.01%、22.31%（P〈0.05）;Ig G含量分别提高15.19%、20.50%、19.43%（P〈0.05）;Ig M含量分别提高25.67%、20.49%、15.11%（P〈0.05）。3%添加组促卵泡雌激素（FSH）和孕酮（P4）含量分别提高17.90和22.09%（P〈0.05）;试验Ⅰ、Ⅱ、Ⅲ组黄体生成素（LH）含量分别提高23.81%、17.46%、19.05%（P〈0.05）;3%和5%添加组雌二醇（E2）含量分别提高19.96%、11.89%（P〈0.05）。试验Ⅰ、Ⅱ、Ⅲ组大肠杆菌数量分别降低了30.77%、39.74%、37.82%（P〈0.01）;而对乳酸杆菌数量影响不大（P〉0.05）。综上所述,松针粉可以提高柴鸡血清免疫球蛋白和生殖激素的水平,改善肠道微生物菌群。综合考虑,确定松针粉在柴鸡日粮中的最适添加量为3%。The objective of this study was to evaluate the effects of pine needle powder on serum immunoglobulin, re- productive hormone and intestinal flora of free-range layers.A total of 120 free-range layers aged 160 days were randomly divided into 4 groups with 3 replicates of 10 each.The layers were fed a basal diet containing 0%,3%,5%,7% pine needle powder.The results showed as follows：compared with control,lgA content of group Ⅰ, Ⅱ and Ⅲ increased by 23.12%, 18.01% and 22.31%,respectively （P 〈 0.05）;IgG content increased by 15.19%,20.50% and 19.43%,respectively （P 〈 0.05） ;IgM content increased by 25.67%, 20.49% and 25.67% ,respectively （P 〈 0.05）.The fallicle-stimulating normone （FSH） level and progesterone （P4） level improved significantly by 17.90% and 22.09%（P 〈 0.05） when pine needle pow- der adding level was 3%（P 〈 0.05）;the luteinizing hormone （LH） levels increased by 23.81%, 17.46% and 19.05% ,re- spectively （P 〈 0.05） ;the estradiol （E2） levels improved significantly by 19.96% and 11.89% when pine needle powder adding level was 3% and 5% respectively （P 〈 0.05）.The number of Escherichia coli was reduced by 30.77%, 39.74% and 37.82% respectively （P 〈 0.01 ） ;there was no significant differences among groups on the number of Lactobacillus （P 〉 0.05）.In summary,serum immunoglobulin,reproductive hormones,and intestinal flora of free-range layers were improved by adding pine needle powder and 3% adding level of pine needle powder was recommended.

关 键 词：柴鸡 松针粉 免疫球蛋白 生殖激素 肠道菌群 

分 类 号：S816.7[农业科学—饲料科学]