HPLC手性固定相法拆分YNC光学异构体  

Chiral separation of YNC and its optical isomers by HPLC

作　　者：尹文龙[1] 郝英魁[2] 全红娜 潘雁涛 蒋庆峰[2] 

机构地区：[1]天津中医药大学,天津300193 [2]天津药物研究院药物分析测试中心,天津300193 [3]中国科学器材公司,北京100028

出　　处：《现代仪器与医疗》2015年第2期61-63,共3页Modern Instruments & Medical Treatment

摘　　要：目的:建立YNC 3个异构体的HPLC拆分法。方法:采用CHIRALPAK AD-H柱(4.6×250mm,5μm),以正己烷-异丙醇(82:18)为流动相,检测波长232 nm。结果与结论 :在选定色谱条件下YNC与其3个异构体完全分离,可用于本品的质量控制。Objective： A chiral HPLC method was established for the separation and determination of YNC and its three optical isomers. Method： Chiral separation of optical isomers was achieved under normalphase chromatographic mode by the Chiralpak AD-H chiral stationary phase. The optimized chromatographic conditions were a mobile phase consisting of n-hexane-isopropanol（82：18）, a fl ow rate of 1 m L/min and an UV detector at 232 nm. Results and conclusion： Under the selected chromatographic conditions, complete separation of ezetimibe and its thee isomers can be applied for quality control of the product.

关 键 词：YNC 异构体 高效液相色谱 手性固定相 

分 类 号：R917[医药卫生—药物分析学]