多药耐药肺炎克雷伯菌毒力基因研究  

Investigation on virulence genes in multidrug-resistant Klebsiella pneumoniae

作　　者：姜如金[1] 朱健铭 翁幸鐾[2] 吴康乐[1] 孔海深[3] 

机构地区：[1]杭州市余杭区中医院检验科,浙江杭州311106 [2]宁波市第一医院检验科,浙江宁波315010 [3]浙江大学医学院附属第一医院传染病诊治国家重点实验室,浙江杭州310003

出　　处：《中华医院感染学杂志》2015年第7期1441-1443,共3页Chinese Journal of Nosocomiology

基　　金：国家"十一五"科技重大专项基金资助项目(2008ZX100-03-004);杭州市科技计划引导基金项目(2012027)

摘　　要：目的了解多药耐药肺炎克雷伯菌(MDRKP)中5种毒力基因存在情况,为临床治疗提供参考依据。方法收集2008年8月-2010年5月杭州市和湖州市6所医院共47株MDRKP,采用PCR及序列分析方法检测5种毒力基因(arr、magA、rmpA、hofA和ompT)。结果 47株MDRKP中检测到arr和magA两种毒力基因,检出率分别为29.8%和17.0%;6所医院A、B、C、D、E、F的MDRKP分离株中毒力基因arr的检出阳性率分别为26.7%、81.8%、0、14.3%、0、0,6所医院A、B、C、D、E、F的MDRKP分离株中毒力基因magA的检出阳性率分别为0、0、0、0、0、88.9%。结论 MDRKP中毒力基因arr和magA具有一定的携带率,在MDRKP中检出毒力基因arr和magA为国内首次报道。OBJECTIVE To investigate the distribution of 5 kinds of virulence genes in 47 strains of multidrug-resistant K lebsiella pneumoniae （MDRKP） ,so as to provide reference for clinical treatment .METHODS Totally 47 strains of MDRKP from Aug .2008 to May 2010 were collected from 6 hospitals in Hangzhou and Huzhou in Zhejiang Province in China .Then ,5 kinds of virulence genes（arr ,magA ,rmpA ,hofA ,ompT） were analyzed by PCR .RESULTS In 47 strains of MDRKP ,2 kinds of virulence genes （arr ,magA）were detected ,and the detection rates were 29 .8% （14/47）and 17 .0% （8/47） .The detection rates of arr in hospitals A ,B ,C ,D ,E ,F were 26 .7% ,81 .8% ,0 ,14 .3% ,0 ,0 ,respectively .The detection rates of magA in hospitals A ,B ,C ,D ,E , F were 0 ,0 ,0 ,0 ,0 ,88 .9% , respectively .CONCLUSION Virulence genes （arr , magA ）were positive in MDRKP .And this is the first report that virulence genes（arr ,magA）were positive in MDRKP .

关 键 词：肺炎克雷伯菌 多药耐药 毒力基因 

分 类 号：R378.996[医药卫生—病原生物学]