纳米银对阿萨希毛孢子菌抑菌效果及作用机制的研究  

The antifungal properties and mechanisms of silver nanoparticles on Trichosporon asahii

作　　者：夏志宽[1] 夏建军[2] 陈卫[1] 丛林[1] 张德全[1] 杨蓉娅[1] 

机构地区：[1]北京军区总医院皮肤科,北京100700 [2]北京军区北戴河疗养院第三疗养区,河北秦皇岛066100

出　　处：《中华医院感染学杂志》2015年第7期1483-1486,共4页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金资助项目(81271764)

摘　　要：目的明确并评价纳米银对阿萨希毛孢子菌的抑菌效果,探讨其可能的作用机制,为寻找新的抗真菌药物提供理论依据。方法观察不同浓度纳米银在马铃薯葡萄糖琼脂培养基（PDA）上阿萨希毛孢子菌的生长情况,用M-27标准方案测定其MIC值,透射及扫描电镜下观察纳米银对阿萨希毛孢子菌超微结构的影响。结果发现纳米银对阿萨希毛孢子菌具有明显的抑制作用,且与浓度呈正相关,菌落直径为0-5.5cm;其MIC值为0.5μg/ml,大于伏立康唑的0.03-0.06μg/ml,小于其他常用抗真菌药物;电镜下发现,纳米银对其细胞壁及细胞膜均有不同程度的破坏,对线粒体、染色质、核糖体等细胞器结构的破坏也非常明显。结论纳米银对阿萨希毛孢子菌生长具有明显抑制作用,其抗菌效果好于常用的抗真菌药物,其作用机制可能为通过对细胞壁、细胞膜的破坏或者直接渗透入细胞内,引起细胞内容物外泄及细胞器的损伤,最终可导致细胞死亡。OBJECTIVE To evaluate the antifungal properties of silver nanoparticles against the pathogenic fungus Trichosporon asahii,and explore its possible mechanisms so as to provide theoretical basis for new antifungal drugs.METHODS The growth of T.asahii on potato dextrose agar（PDA）media containing different concentrations of silver nanoparticles was examined and the antifungal effect of silver nanoparticles was evaluated using minimum inhibitory concentration（MIC）.Scanning and transmission electron microscopy（SEM/TEM）were also used to investigate the antifungal effect of silver nanoparticles on T.asahii.RESULTS Silver nanoparticles had a significant inhibitory effect on the growth of T.asahii.And the result was in proportional to the concentration of silver nanoparticles.The colony diameter was 0to 5.5cm.The MIC value of T.asahii was 0.5μg/ml,which was higher than voriconazole 0.03-0.06μg/ml but lower than other common antifungal drugs.Silver nanoparticles were found harm to cell wall and cell membrane under the electron microscope and they also showed an obvious damage to the mitochondria,chromatin and ribosome.CONCLUSION Our results demonstrated that silver nanoparticles,rather than other antifungal drugs,have shown remarkable antifungal effect against T.asahii.The SEM/TEM observation suggested that silver nanoparticles inhibit the growth of T.asahii by means of permeating the fungal cell and damaging the cellular components,causing cellular content escape and damaging organelle and leading to the death of cells as a result.

关 键 词：纳米银 阿萨希毛孢子菌 抗真菌作用 超微结构 

分 类 号：R378[医药卫生—病原生物学]