人胚胎干细胞来源的肝细胞小鼠移植的实验研究  

An experimental study on human embryonic stem cell-derived hepatocytes transplantation into liver decompensated mice

作　　者：梁道明[1] 张毅[1] 罗敏[2] 段永庆[1] 周粼[1] 王华[1] 

机构地区：[1]昆明医科大学第二附属医院普通外科,650101 [2]昆明医科大学云南省天然药物药理重点实验室

出　　处：《中华普通外科杂志》2015年第3期211-214,共4页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目（81060114）

摘　　要：目的 将人胚胎干细胞诱导分化成的肝细胞移植入小鼠模型,探讨其细胞整合到肝脏的能力,并观察对肝脏细胞损害的修复作用.方法 将人胚胎于细胞诱导分化成的肝样细胞移植入慢性肝功能衰竭小鼠体内l、7、15 d后,检测小鼠的肝脏病理组织学、肝功能以及分化肝细胞参与受体肝组织的再生及分化为成熟的肝细胞的情况.结果 移植后肝脏组织病理变化明显改善,表现为肝细胞以变性和炎性细胞浸润为主,坏死灶小、充血以及出血现象减轻;同时肝功能好转.分离到的人胚胎干细胞可在肝受损小鼠体内存活,增殖,整合,并能分化为成熟的肝细胞.结论 将人胚胎干细胞移植入小鼠体内后参与受体肝组织的再生,并能分化成功能性的肝细胞.Objective To study the ability of human embryonic stem cells to integrate into mouse liver and to repair chronic liver injury of the recipient.Methods On day 1,day-7 and day-15 after human embryonic stem cells were induced to differentiate into hepatocyte-like cells and were transplanted into mice with chronic liver failure,liver histopathology,liver function,liver tissue regeneration and mature hepatocytes of mice were tested respectively.Results Hepatic tissue pathology of mice significantly improved after transplantation and necrotic foci diminished,hemorrhage and congestion of hepatic cells relieved,and liver function improved.It was observed that human embryonic stem cells survived,proliferated,integrated with host liver,and differentiated into mature hepatocytes.Conclusions Human embryonic stem cells xenotransplanted into mice can participated liver tissue regeneration to some extent,and differentiated into functional liver cells.

关 键 词：胚胎干细胞 干细胞移植 动物实验 

分 类 号：R285.5[医药卫生—中药学]