巴西橡胶树HbMKK4基因的克隆及表达分析  

Molecular Cloning and Expression of HbMKK4 from Hevea brasiliensis(Para Rubber Tree)

作　　者：吴绍华[1] 张世鑫[1,2] 杨署光[1] 田维敏[1] 

机构地区：[1]中国热带农业科学院橡胶研究所、农业部橡胶树生物学与遗传资源利用重点实验室、省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室,海南儋州571737 [2]海南大学园艺园林学院,海口570228

出　　处：《西北植物学报》2015年第3期440-446,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家天然橡胶产业技术体系(CARS-34-GW1);国家自然科学基金(31300504)

摘　　要：采用RACE技术,从橡胶树‘热研7-33-97’中克隆了1个促分裂原活化蛋白激酶的激酶(MKK)基因HbMKK4。该基因全长cDNA序列1 580bp,编码框1 059bp,编码352个氨基酸,含有S_TKc结构域,其编码蛋白的分子量为38.83kD,理论等电点为9.36。实时荧光定量PCR结果显示,HbMKK4在橡胶树的根、树皮、胶乳及叶片中均有表达。割胶、茉莉酸甲酯和乙烯利均能上调胶乳中HbMKK4基因的表达,基因相对表达量分别在割胶后2h、茉莉酸甲酯处理8h和乙烯利处理4h后达到最高。研究结果推测HbMKK4可能通过MAPK信号途径参与茉莉酸信号途径的响应,可能在天然橡胶生物合成调控中起关键作用。In this work,the full length cDNA of HbMKK4 was obtained from cDNA library in latex of rubber tree clone‘CATAS 7-33-97＇by RACE.It was 1 580 base pair（bp）in length,containing a 1 059 bp open reading frame（ORF）which encodes 352 amino acid with a predicted molecular mass of 38.83 kD and pI 9.36.The deduced amino acid sequence contain S_TKc domain.Comparing expression among different tissues showed that HbMKK4 was expressed in root,bark,latex and leaves.HbMKK4 transcript levels were significantly upregulated in latex by tapping,methyl jasmonate and ethephon treatment for rubber tree.The transcript levels peaked in 2hours by tapping,8hours by methyl jasmonate and 4hours by ethephon treatment,respectively.These results suggested that HbMKK4,a member of MAPK cascade,might plays a role in rubber biosynthesis via JA pathway in rubber tree.

关 键 词：巴西橡胶树 HbMKK4 基因克隆 基因表达分析 

分 类 号：Q786[生物学—分子生物学]