蚯蚓冻干粉对高血脂小鼠胆固醇逆转运相关基因的调节作用  

Regulation of earthworm lyophilized powder on reverse cholesterol transport genes expression in hyperlipidemic mice

作　　者：武金霞[1] 武建[1] 隋思强 王静之[1] 张贺迎[2] 

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]河北大学生物工程技术研究中心,河北保定071002

出　　处：《河北大学学报（自然科学版）》2015年第2期159-164,共6页Journal of Hebei University(Natural Science Edition)

基　　金：河北省生物工程重点学科资助项目

摘　　要：以高脂饲料饲喂昆明种小鼠构建高血脂小鼠模型,灌胃给予高、中、低剂量(0.4,0.2,0.1g/kg)蚯蚓冻干粉,每天1次,同时饲喂高脂饲料,以仅饲喂高脂饲料组小鼠为对照,共饲养10周.提取小鼠肝脏总RNA,应用半定量RT-PCR技术检测实验组与对照组小鼠胆固醇逆转运相关基因卵磷脂胆固醇酰基转移酶(lecithin cholesterol acyltransferase,LCAT)和脂蛋白酯酶(lipoprotein lipase,LPL)mRNA表达水平.结果表明,蚯蚓冻干粉极显著提高实验组小鼠LCAT mRNA表达水平(P<0.01),显著提高LPL mRNA表达水平(P<0.05),提示这是蚯蚓冻干粉能够降低高血脂小鼠血浆胆固醇水平的原因之一.Kunming mice were fed with high-level lipid feeds to establish the hyperlipidemic mice models. The mice were treated with earthworm lyophilized powder at dosages of 0.4, 0.2 and 0.1 g/kg once a day and were fed with high-level lipid feeds at the same time for 10 weeks, the mice fed only with high level lipid feeds were as the control. The total RNA of liver was extracted, and the changes of liver lecithin cholesterol acyltransferase and lipoprotein lipase gene expression were analyzed by RT PCR. The results indicated that earthworm lyophilized powder up-regulated lecithin cholesterol acyltransferase and lipoprotein lipase mRNA expression markedly, suggesting it was one of the reasons for reducing serum cholesterol in hyperlipidemic mice of earthworm lyophilized powder.

关 键 词：蚯蚓冻干粉 高血脂症 胆固醇代谢 卵磷脂胆固醇酰基转移酶 脂蛋白酯酶 

分 类 号：Q507[生物学—生物化学]