沈阳森林动物园丹顶鹤核心种群的遗传多样性分析  

Analysis of Genetic Diversity in the Red-crowned Crane Population at Shenyang Forest Zoo

作　　者：张佳佳[1] 田秀华[1] 宋艳珠 董海燕[1] 白素英[1] 张玥[1] 

机构地区：[1]东北林业大学野生动物资源学院,哈尔滨150040 [2]沈阳森林动物园,沈阳110000

出　　处：《野生动物学报》2015年第1期58-62,共5页CHINESE JOURNAL OF WILDLIFE

摘　　要：利用7个SSR位点对沈阳动物园丹顶鹤核心种群进行了遗传多样性分析,统计了等位基因数(N_a)、有效等位基因数(N_c),计算了群体杂合度(H_e)、多态信息含量(PIC)、Shannon信息指数(I)、近交系数(F_(is))等参数。结果显示:7个微卫星标记中有4个高度多态位点和3个中度多态位点,共扩增出36个等位基因,并发现了11个稀有等位基因;平均有效等位基因是2.4342,平均期望杂合度为0.5510,平均多态信息含量是0.5049,遗传多样性水平较高;哈温平衡检测显示5个位点符合哈温平衡,2个位点偏离了哈温平衡;平均F_(is)值为0.0069,同时平均观测杂合度小于平均期望杂合度,显示可能存在种群个体亲缘关系较近的情况。We analyzed the genetic diversity of the red -crowned crane（ Grus japonensis）popula- tion at Shenyang Forest Zoo by using 7 SSR loci. We counted numbers of alleles（ Na ）and effec- tive numbers of alleles （ Ne ）, and calculated polymorphism information content （ PIC）, Shannon information index（I） and inbreeding coefficient（F^s）. There were 4 highly polymorphic loci and 3 moderately polymorphic loci, 36 alleles were amplified, and 11 rare alleles were found. The aver- age Newas 2. 4342, the average expected heterozygosity was 0. 5510, and the average PIC was 0. 5049, showing higher levels of genetic diversity, Hardy - Weinberg equilibrium （HWE） testing showed that 5 SSR loci were in accord with HWE and 2 loci were not in HWE. Mean Fis wasO. 0069, while the average expected heterozygosity was greater than the average observed het- erozygosity, indicating that there were closer relationships between some individuals.

关 键 词：丹顶鹤 遗传多样性 SSR 

分 类 号：Q958.1[生物学—动物学]