高龄肝脏与低龄肝脏生理差异的实验性研究  

Experimental of physiological differences between elderly and younger livers in rats

作　　者：黄宇[1] 黄迪[1,2] 翁杰锋[1,2] 张帅[1,2] 张强[1] 古维立[1,2] 

机构地区：[1]广州医科大学附属广州市第一人民医院肝胆外科,广州510180 [2]广州消化疾病中心,广州510180

出　　处：《广州医药》2015年第2期7-12,共6页Guangzhou Medical Journal

基　　金：广东省科技计划项目(2011B061300024;2013B021800057);广东省自然基金项目(10151006001000013);广州市医药卫生科技一般引导项目(20131A011029;20141A010003);广州市科技和信息化局科普专项项目(2014KP000086)

摘　　要：目的探讨生理情况下高龄肝脏与低龄肝脏的区别,寻找可以区分高龄肝脏与低龄肝脏的指标。方法取6周龄SD大鼠和9月龄以上退役SD大鼠各5只,采用超声弹性成像检测肝脏硬度、全自动生化检测仪检测血清学指标、H&E染色观察肝脏形态结构、Sirius Red染色及Masson染色检测胶原纤维的沉积、免疫组化SP法检测TGF-β1、p16INK4a、SMP-30蛋白的表达。结果高龄组和低龄组之间血清学指标、胶原纤维沉积及TGF-β蛋白、p16INK4a蛋白的表达无差异;超声弹性成像检测低龄组Vs值为(1.21±0.09)m/s,高龄组为(1.32±0.05)m/s(P=0.033);SMP-30蛋白低龄组IOD值为138244.988±51286.257,高龄组为116240.170±35017.936(P=0.007)。结论高龄大鼠与低龄大鼠肝脏的硬度及SMP-30蛋白的表达存在差异,随着年龄的增加肝脏硬度增大,SMP-30蛋白表达下降。肝脏硬度与SMP-30蛋白可作为区分高龄肝脏与低龄肝脏的指标。Objective To investigate the differences between elderly and younger liver. Methods In accordance with the age of the SD rats into two groups ： younger group （ Group Y, 6 weeks, n = 5） and elderly group （ Group 0, 40 weeks or more, n = 5 ） . Data were compared by using ultrasound elasticity imaging to detect liver stiffness, automatic biochemical detec- tor to gauge serum indexes, H＆E staining to observe the liver morphological structure, Sirius Red staining and Masson staining to assay the collagen fibers deposition, Immunohistoehemistry to identify the expression of TGF-β1, p16^INK4a and SMP-30 protein. Results Serum indexes, collagen deposition, TGF-β1 and p16^INK4a protein expression were no statistically significant difference between two groups. The Vs value was （ 1.21 ± 0. 09） m/s in Group Y and （ 1.32 ± 0. 05 ） m/s in Group O （ P = 0. 033 ） . and the IOD value of SMP-30 protein between Group Y and Group O were 138244. 988 ±51286. 257 and 116240. 170 ±35017. 936 （P = 0. 007 ） . Conclusion The degree of liver stiffnessnd and SMP-30 protein in elderly and younger liver are differ- ent. Increased the degree of liver stiffness and decreased the expression of SMP-30 protein in the elderly SD rats. Liver stiffness and SMP-30 protein could be used as indicators to distinguish between elderly and younger liver.

关 键 词：高龄 肝移植 SMP-30 超声弹性成像 

分 类 号：R333.4[医药卫生—人体生理学]