黄连素通过抑制PLCγ1活性阻止肝癌细胞的侵袭  

Inhibitory Effects of Berberine on Invasion in Hepatocellular Carcinoma Cells by Down-regulating PLCγ1

作　　者：王娟[1] 谢波[1] 刘英[1] 陈萍[1] 汪选斌[1] 李洪亮[1] 刘明[1] 曹风军[1] 

机构地区：[1]湖北医药学院附属人民医院中药药理实验室,湖北十堰442000

出　　处：《湖北医药学院学报》2015年第1期23-25,30,F0002,共5页Journal of Hubei University of Medicine

基　　金：湖北省科技厅省自然基金项目(2010CDZ066;2014CFB642);湖北省卫生计生科研基金资助项目(WJ2015MB292);湖北省2011协同创新中心基金(项目编号:4);湖北省十堰市2014科技计划项目(14K66);湖北医药学院优秀中青年团队项目(2014CXG03);湖北医药学院教学研究立项项目(2013029);湖北省重点学科项目(2014XKJSXJ18)

摘　　要：目的:研究黄连素(Berberine,BBR)对人肝癌细胞侵袭的阻止作用,并探讨其机制是否与抑制PLCγ1有关。方法:人肝癌SMMC-7721消化接种于用Matrigel胶包被的Transwell小室,在不同浓度的黄连素及JX401(p38抑制剂)、U0126(Erk抑制剂)、SU5416(VEGF抑制剂)作用24 h后,结晶紫染色并计数Transwell下室的细胞数;用Western-blot法检测不同浓度黄连素作用下p-PLCγ1的表达。结果:BBR对肝癌细胞SMMC-7721的侵袭有明显阻止作用,与SU5416(VEGF抑制剂)作用类似。Westernblot结果显示黄连素抑制了SMMC-7721的p-PLCγ1表达。结论:黄连素具有阻止肝癌细胞侵袭的作用,其机制可能与抑制肿瘤侵袭相关信号VEGF/PLCγ1的活性有关。Objective To investigate the inhibitory effects of berberine（ BBR） on invasion of hepatocellular carcinoma cells,and explore whether the effects was associated with inhibition of PLCγ1. Methods Human hepatocellular carcinoma cells SMMC-7721 were digested and sub-cultured in the transwell chambers coated by Matrigel,and BBR,JX401（ p38 inhibitor）,U0126（ Erk inhibitor）,SU5416（ VEGF inhibitor） were added into down chambers for 24 h. The cells in the down chambers were stained by crystal violet and counted. The expression of p-PLCγ1 was determined by Western-blot after cocultured with different concentration BBR. Results BBR significantly inhibited the invasion of SMMC-7721 cells,and the effect of BBR was similar to SU5416（ VEGF inhibitor）. The expression of PLCγ1 was inhibited by BBR in SMMC-7721 cells. Conclusion Berberine can inhibit the invasion of human hepatocellular carcinoma cells,which may be related with inhibiting activation of VEGF / PLCγ1.

关 键 词：黄连素 人肝癌细胞 侵袭 磷脂酶Cγ1 

分 类 号：R285[医药卫生—中药学]