静脉注射内吗啡肽对麻醉大鼠左心室功能的影响  

Effect of endomorphin-1 and endomorphin-2 on the left ventricular function in anesthetized rats

作　　者：宗巧凤 张冠军[1] 于影[1] 李正红[1] 

机构地区：[1]蚌埠医学院生理学教研室,安徽蚌埠233000

出　　处：《皖南医学院学报》2015年第1期6-9,共4页Journal of Wannan Medical College

基　　金：安徽省高校省级自然科学研究重点项目(KJ2011A202);蚌埠医学院研究生创新计划项目(BYYCX1307)

摘　　要：目的:观察静脉注射内吗啡肽-1和2(EM-1、EM-2)对大鼠左心室功能的影响,并初步探讨其作用机理。方法:大鼠麻醉后,经右颈总动脉左心室插管测左心室功能(LVSP、HR、±dp/dt等)。颈外静脉注射给药。结果:与对照组比较,静脉注射EM-1、EM-2剂量依赖性地降低麻醉大鼠左心室功能。与EM-1/EM-2+NS组比较,除一氧化氮合成酶抑制剂L-NNA(25 mg/kg,i.v.)对EM-1降低心率的作用无明显影响外,预先给予纳洛酮(1 mg/kg,i.v.)或阿托品(50μg/kg,i.v.)或L-NNA(25 mg/kg,i.v.),或切断双侧迷走神经均显著减弱EM-1、EM-2降低心功能的作用(P<0.05或P<0.01)。结论:静脉注射EM-1、EM-2可引起麻醉大鼠左心室功能下降,此效应由阿片受体介导,有胆碱能M受体参加,有NO的参与,与迷走神经兴奋有关。Objective： To observe the effects of intravenous injection of endomorphin-1（ EM-1） and endomorphin-2（ EM-2） on the left ventricular function in anesthetized rats,and to primarily investigate its potential mechanism. Methods： The left ventricular function（ LVSP,HR,± dp / dt） were measured by inserting a catheter from right carotid artery into left ventricle after intravenous injection of EM-1 and EM-2 in the anesthetized rats. Results： Compared with control group,EM-1 and EM-2 led to decreased dose-dependent for the left ventricular function. By comparison with EM ＋ NS group,the decrease in left ventricular function was attenuated by pretreatment with intravenous injection of either naloxone（ 1 mg / kg,i. v.）,atropine（ 50 μg / kg,i. v.）,L-NNA（ 25 mg / kg,i. v.） or bilateral cervical vagotomy. Conclusion： Intravenous administration of EM-1 and EM-2 produced inhibition effect on the left ventricular function in anesthetized rats,and the potential mechanism is associated with mediation of opioid receptor,involvement of M-cholinoceptor and NO as well as vagus nerve excitation.

关 键 词：内吗啡肽 阿片受体 左心室功能 

分 类 号：R331[医药卫生—人体生理学] R961[医药卫生—基础医学]