双组分信号转导系统SrrBA调控表皮葡萄球菌生长和生物膜形成  

Two-component signal transduction system SrrBA to regulate bacterial growth and biofilm formation in Staphylococcus epidermidis

作　　者：朱涛[1] 谷生丽[1] 

机构地区：[1]皖南医学院医学寄生虫学教研室,安徽芜湖241002

出　　处：《皖南医学院学报》2015年第1期12-16,共5页Journal of Wannan Medical College

基　　金：皖南医学院博士科研启动基金项目(2011)

摘　　要：目的:探讨双组分信号转导系统SrrBA在表皮葡萄球菌中的调控作用。方法:利用同源重组技术构建表皮葡萄球菌srrBA基因敲除突变株,通过检测OD600值绘制其有氧生长曲线,并观察其厌氧生长状态,微量板半定量方法检测其生物膜形成能力。结果:经PCR扩增和测序验证获得了表皮葡萄球菌srrBA基因敲除突变株(SE 1457-ΔsrrBA)。突变株的生长不论是在有氧还是在厌氧条件下均明显滞后于野生株。此外,突变株形成生物膜的能力较之野生株也显著下降。结论:双组分系统SrrBA可调控表皮葡萄球菌的生长和生物膜形成。Objective： To explore the regulatory role of the two-component signal transduction system SrrBA in Staphylococcus epidermidis（ S. epidermidis）.Methods： Homologous recombination technique was used to generate a srrBA knockout mutant in S. epidermidis 1457 via temperature-sensitive shuttle vector pMAD. Bacterial growth curve was determined by measuring the value of OD600 under aerobic condition,with anaerobic growth being observed. Effect of srrBA mutant on the biofilm formation was measured by a semi-quantitative microtiter plate assay. Results： The knockout mutant was successfully acquired and verified by PCR amplification and sequencing. The mutant exhibited drastically retarded growth compared to the parent strain under either aerobic or anaerobic conditions. Moreover,biofilm formation was significantly reduced in the srrBA mutant. Conclusion： The SrrBA two-component system could regulate bacterial growth and biofilm formation in S. epidermidis.

关 键 词：双组分信号转导系统 SrrBA 表皮葡萄球菌 生物膜 

分 类 号：R378[医药卫生—病原生物学]