一株苯酚菌的鉴定及其降解特性研究  

Identification and Characteristics of AHigh Phenol-degrading Bacterial Strain

作　　者：郑贝贝[1] 霍莹[1] 杨勇[1] 付连超 张莹[1] 

机构地区：[1]中海油天津化工研究设计院,天津300131

出　　处：《广州化工》2015年第7期95-97,共3页GuangZhou Chemical Industry

摘　　要：以苯酚为唯一碳源筛选分离纯化降解苯酚菌株;通过形态观察、16S rRNA序列分析对其进行初步鉴定,利用4-氨基安替比林分光光度法测定菌株在不同温度、p H、及盐含量条件下的降酚能力。结果表明,筛选分离出的C3菌株为红球菌属（Rhodococcus sp.）细菌;菌株C3的最佳降酚温度为35℃、最佳p H值为8.0、最佳盐含量范围为0~2%,在最适宜条件下,菌株C3可在28 h内将600 mg/L苯酚完全降解,对浓度高达1000 mg/L的苯酚具有一定的降解能力。In order to screen high phenol-degrading bacterial strains, bacteria was acclimated successively by the inorganic salt medium containing phenol as a sole carbon source. Phenol- degrading bacterial strain was identified preliminarily by its morphological and 16S rRNA sequences. The phenol degradation ability under different temperature, pH, salt content, and optimal condition was determined by 4-AAP spectrophotometric method. The results showed that the strain C3 was identified as Rhodococcus sp.. The optimal temperature of phenol degradation was 35 ℃, the optimal pH value was 8.0, and the optimal salt content was 0 -2%. Under optimal conditions, it could completely degrade phenol of 600 mg/L in 28 h, and it could degrade phenol of 1000 mg/L partly as well.

关 键 词：红球菌属 苯酚 生物降解 

分 类 号：X522[环境科学与工程—环境工程]