星点设计—效应面法结合双指标优化超声提取黄芩中黄酮苷类成分  

Optimization of Ultrasonic Extraction Technology of Flavonoid Glycosides in Scutellaria Baicalensis by the Combination of CCD-RSM and a Two-indicator Model

作　　者：苏建春[1,2] 柯华香[2,3] 马莉[4] 赵俊霞[1] 孙彩霞[1] 尹蓉莉[1] 李化[2,5] 

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]中国中医科学院中药研究所,北京100700 [3]西华大学生物工程学院,四川成都610039 [4]首都医科大学中医药学院,北京100069 [5]中国中医科学院道地药材国家重点实验室培育基地,北京100700

出　　处：《亚太传统医药》2015年第7期32-36,共5页Asia-Pacific Traditional Medicine

基　　金：国家自然科学基金项目(81202903);科技部中医药行业科研专项(201407003);北京市属高等学校高层次人才引进与培养计划项目(CIT&TCD201304179);国家药典委员会科研课题("中药材品种限制产地和生长年限的研究和制定")

摘　　要：目的:采用星点设计—效应面法优化黄芩中黄酮苷类成分的提取工艺。方法:采用UPLC法测定黄芩苷及汉黄芩苷含量,以二者的总评"归一值(OD)"为因变量,乙醇浓度、提取时间、料液比为自变量,对结果进行多元线性回归和二项式方程拟合,采用效应面法优化提取工艺,并进行预测分析。结果:最佳提取工艺为58%乙醇,超声提取45min,料液比为1∶40,OD预测值(0.997)与验证值(0.991)的偏差小于1%。结论:星点设计—效应面法优选的黄芩中黄酮苷类成分的超声提取工艺简便,模型预测性良好,可为探讨黄芩中苷类成分提取工艺的研究提供参考。Objective：To optimize ultrasonic extraction Technology of baicalin and wogonoside in Scutellaria baicalensis by central composite design-reponse surface method（CCD-RSM）.Methods：The contents of baicalin and wogonoside were determined by Ultra performance liquid chromatography（UPLC）.Independent variables were ethanol concentration,liquid-solid ratio and extraction time,the dependent variable was the overall desirability of extraction rate of baicalin and wogonoside.Linear or no-linear mathematic models were established to estimate the relationship between independent and dependent variables.Response surface methodology was used to optimize the process of extraction.Prediction was carried out through comparing the observed and predicted values.Results：The optimum extraction technology was as following：The extraction time was 45 min with solvent to solid ratios（v/w）of 40：1,and the extraction solvent was 58% ethanol at the extraction temperature of 40℃.The deviation between the observed and predicted values was small（P〈0.01）.Conclusion：This optimized extraction technology was stable and feasible with high predictability.This method can be provided reference for exploring the flavonoid glycosides in Scutellaria baicalensis.

关 键 词：黄芩 黄芩苷 汉黄芩苷 星点设计—效应面法 超声提取 

分 类 号：R284[医药卫生—中药学]