F^-和Cl^-对莱茵衣藻的生长效应  

Effects of F^- and Cl^- on Growth of Chlamydomonas reinhardtii

作　　者：李潜[1] 吴沿友[2,3] 梁永杰[1] 吴运东[3] 朱红力[1] 

机构地区：[1]江苏大学环境与安全工程学院,江苏镇江212013 [2]中国科学院地球化学研究所环境地球化学国家重点实验室,贵州贵阳550002 [3]江苏大学农业工程研究院,江苏镇江212013

出　　处：《生态与农村环境学报》2015年第2期238-243,共6页Journal of Ecology and Rural Environment

基　　金：国家自然科学基金(40973060)

摘　　要：为了探讨和比较F-和Cl-对莱茵衣藻(Chlamydomonas reinhardtii)生长的抑制毒性,采用锥形瓶培养法及模型分析,研究了不同浓度F-和Cl-胁迫下莱茵衣藻生物量、比生长速率及生长抑制程度的变化。结果表明:5~80mg·L-1的F-对莱茵衣藻均具生长抑制毒性,且抑制率随F-浓度和暴露时间的增加而增大,但10 d最大抑制率低于62%,因此,莱茵衣藻对80 mg·L-1以下的F-具有较好的耐受性;Cl-对莱茵衣藻的生长既有促进作用,甚至也有严重的抑制作用(12 800 mg·L-1Cl-处理组10 d抑制率可高达96%),这取决于Cl-浓度;F-和Cl-对莱茵衣藻的生长效应不同,主要表现在生长效应模型系数随F-和Cl-浓度的变化规律上。另外,就相同暴露时间下的EC50值而言,F-(18.0~56.4 mg·L-1)远低于Cl-(1 074~2 630 mg·L-1),即F-对莱茵衣藻的毒性高于Cl-,但Cl-的生态风险亦不容忽视。To explore toxic effects of F-and Cl-on growth of Chlamydomonas reinhardtii for comparison,algal biomass,specific growth rate and growth inhibition rate as affected by concentration of F-or Cl-were determined with the Erlenmeyer flask cultivation method and model analysis. Results show that F-in the range of 5- 80 mg·L-1was toxic to C. reinhardtii,inhibiting its growth and the growth inhibition rate increased with increasing exposure time and F-concentration,but its maximum growth inhibition rate dropped below 62% on D 10,which suggests that C. reinhardtii is quite tolerable to F-below 80 mg·L-1in concentration. By contrast,Cl-could either promote or inhibit growth of C. reinhardtii depending on Cl-concentration. When Cl-concentration was up to 12 800 mg · L-1,its growth inhibition rate reached as high as96%. F-and Cl-differs in effect on growth of C. reinhardtii,which is reflected mainly in the laws of the functions of the growth effect models varying with F-or Cl-concentration. Besides,in terms of EC50 under the same exposure time F-（ 18. 0 to 56. 4 mg·L-1） was far too lower than Cl-（ 1 074 to 2 630 mg·L-1）. That is to say,F-is more toxic to C. reinhardtii than Cl-,but the ecological risk of Cl-in the environment may not be ignored,either.

关 键 词：F- Cl- 莱茵衣藻 生长 抑制 模型 

分 类 号：X524[环境科学与工程—环境工程]