Box-Behnken设计-效应面法优化白花蛇舌草总黄酮的提取工艺  

Optimization of Extraction Process of Total Flavonoids from Oldenlandia diffusa by Box-Behnken Designresponse Surface Methodology

作　　者：李芳[1,2] 杨培民[2] 曹广尚[2] 逯双 

机构地区：[1]山东中医药大学药学院,济南250355 [2]山东中医药大学附属医院,济南250011

出　　处：《中国实验方剂学杂志》2015年第8期21-24,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81274052);山东省自然科学基金项目(ZR2011HL043);山东省优秀中青年科学家科研基金项目(BS2013YY052)

摘　　要：目的:优选白花蛇舌草黄酮类成分的提取工艺,为该药材的资源开发提供参考。方法:以乙醇体积分数、乙醇用量及提取时间为自变量,芦丁、异槲皮苷、槲皮苷提取量的总评"归一值"为因变量,通过Design-Expert 8.0软件对自变量各水平进行多元线性回归和二项式拟合,利用效应面法优选提取工艺并进行预测分析。采用HPLC-DAD测定芦丁、异槲皮苷、槲皮苷含量,色谱条件为流动相乙腈(A)-0.05%磷酸溶液(B)梯度洗脱,检测波长254 nm。结果:最佳提取工艺为加14倍量73%乙醇回流提取2次,每次1.5 h;芦丁、异槲皮苷、槲皮苷平均提取量分别为2.529,0.435,0.215 mg·g-1,总评"归一值"实测值0.922 7与预测值偏差-1.24%。结论:优化的提取工艺稳定可行,预测准确度高,为白花蛇舌草总黄酮的制剂开发提供参考。Objective： To provide a reference for resource development of Oldenlandia diffusa by optimize extraction process of total flavonoids from this medicinal herb. Method： Extraction time, ethanol concentration and amount were selected as independent variables, overall desirability of contents of rutin, isoquercitrin and quereitrin as dependent variable, Box-Behnken design-response surface methodology was used to optimize extraction technology. HPLC-DAD was adopted to determine contents of rutin, isoquercitrin and quercitrin with mobile phase of acetonitrile （A） -0. 05% phosphoric acid solution （B） for gradient elution and detection wavelength of 254 nm. Result： Optimal extraction process was as following： extracted 2 times with 14-fold the amount of 73% ethanol for 1.5 h per time. Average extracting amounts of rutin, isoquercitrin and quercitrin were 2. 529, 0. 435, 0. 215 mg · g^-1 , respectively. Deviation between the measured and predicted value of overall desirability was - 1.24%. Conclusion： This extraction process is stable and feasible with high prediction accuracy, which can provide a reference for preparations development of total flavonoids from O. diffusa.

关 键 词：白花蛇舌草 总黄酮 芦丁 异槲皮苷 槲皮苷 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]