总状毛霉对20(S)-原人参二醇的微生物转化及条件优化  

Biotransformation Optimization of 20( S)-protopanaxadiol by Mucor racemosus AS 3.205

作　　者：陈广通[1] 杨雪[1] 葛红娟[1] 李建林[1] 宋妍[1] 

机构地区：[1]南通大学药学院,江苏南通226001

出　　处：《中国实验方剂学杂志》2015年第8期34-38,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81102327)

摘　　要：目的:利用微生物转化的方法构建20(S)-原人参二醇过氧衍生物的新合成途径,并对转化条件进行优化,提高过氧衍生物的产率。方法:选取总状毛霉Mucor racemosus AS 3.205为转化菌株,从接菌量,底物浓度,转化时间,加样时间,温度,转速等方面对转化条件进行优化。结果:获得2个20(S)-原人参二醇过氧衍生物,20(S)-原人参二醇为25,26-烯-24(R)过氧羟基-20(S)-原人参二醇和23,24-烯-25过氧羟基-20(S)-原人参二醇;获得了优化的转化工艺,即接种量为10%,底物浓度为0.25 mmol·L-1,加样时间为转种后48 h,培养温度为28℃,转化时间为5 d,摇床转速为150 r·min-1。结论:该方法能简便的获得20(S)-原人参二醇过氧衍生物,采用优化后的转化条件25,26-烯-24(R)过氧羟基-20(S)-原人参二醇和23,24-烯-25过氧羟基-20(S)-原人参二醇的产率均明显增大。Objective： The aim of this study was to provide a new approach to synthesize hydroperoxylated products of 20 （S）-protopanaxadiol, and to optimize the microbial transformation conditions by Mucor racemosus AS 3. 205. Method： Biotransformation conditions were optimized by altering several influencing factors. The yields of two hydroperoxylated products of 20 （ S）-protopanaxadiol were evaluated. Result： Two metabolites were isolated and identified as 25, 26-en-24 （R） -hydroperoxyl-20 （S） -protopanaxadiol and 23, 24-en- 25-hydroperoxyl-20 （S）-protopanaxadiol. The optimal transformation condition was as following： an inoculation amount at 10% , substrate coneentration at 0.25 mmol ·L^- 1, sample injected after inoculated 48 h, transformation time of 5 d, culture temperature at 28 ℃ , and shaking speed at 150 r · min^-1. Conclusion： Microbial transformation system by M. raeemosus AS 3. 205 can be used to obtain hydroperoxylated products of 20 （ S）- protopanaxadiol. The yields of two hydroperoxylated products could be greatly increased under the optimized condition.

关 键 词：20(S)-原人参二醇 过氧衍生物 微生物转化 总状毛霉AS 3.205 

分 类 号：R284.1[医药卫生—中药学]