一阶导数UV测定独一味及不同提取物中苯乙醇苷含量  

Determination of Phenylethanol Glycosides in Different Extract of Lamiophlomis Herba by First Derivative Ultraviolet Spectrophotometry

作　　者：邱建国[1] 张泉龙[1] 李茂星[1] 贾正平[1] 张汝学[1] 赵一[1] 王谨涵[1] 陶锐[1] 

机构地区：[1]兰州军区兰州总医院,全军高原环境损伤防治重点实验室,国家中医药管理局临床中药学重点学科,兰州730050

出　　处：《中国实验方剂学杂志》2015年第8期43-46,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技重大专项(2008ZXJ09004-XXX);甘肃省科技重大专项(1102FKDA012);甘肃省中医药管理局科研课题(GZK-2014-1);全军中医药研发推广项目(2006032001)

摘　　要：目的：建立独一味水提物、水洗脱物和70%乙醇溶液洗脱物中总苯乙醇苷含量的测定方法。方法：加独一味药材质量18倍量水,煎煮提取3次,每次1.5 h,滤过,合并滤液,滤液2-4℃冷藏除尘,60℃减压干燥得到水提物,水提物经聚酰胺和大孔树脂富集分离,分别用水和70%乙醇溶液洗脱,分别得到水洗脱物,70%乙醇溶液洗脱物Ⅰ（通过聚酰胺柱洗脱物）和70%乙醇溶液洗脱物Ⅱ（通过大孔树脂柱洗脱物）;在224,252,256,354 nm波长处,采用一阶导数紫外分光光度法测定总苯乙醇苷的含量。结果：建立的一阶导数紫外分光光度法测定总苯乙醇苷的含量,在354 nm波长处加样回收率99.71%,RSD 2.5%。结论：该方法专属性强、灵敏度高、精确度与重复性好,适用于独一味中总苯乙醇苷的含量测定。Objective： To determine the total phenylethanol glycosides in water extract, water eluate and 70% ethanol eluate of Lamiophlomis Herba by first derivative ultraviolet spectrophotometry. Method： Lamiophlomis Herba was extracted with water （18 times） 3 times, 1.5 h per time. The water extract was storage at 2- 4 ℃, was dried under reduced pressure at 60 ℃. Water extract were separated and enriched by polyamide and macroporous resin, to obtain the water eluate and 70% ethanol eluate Ⅰ （polyamide column）, 70% ethanol eiuate Ⅱ （macroporous resin column）. The total phenylethanol glycosides were determined by first derivative ultraviolet spectrophotometry at 224, 252, 256, 354 nm. Result ： Recovery of the total phenylethanol glycosides was 99. 71% , RSD was 2.5% at 354 nm. Conclusion： This method is special, sensitive, accurate and repeatable, and can be used for the quality analysis of the total phenylethanol glycosides in Lamiophlomis Herba.

关 键 词：独一味 提取物 一阶导数紫外分光光度法 总苯乙醇苷 

分 类 号：R284.1[医药卫生—中药学]