HPLC测定烈香杜鹃中6种黄酮成分的含量及主成分分析  

Determination of Content of Six Flavonoids in Rhododendron anthopogonoides by HPLC and Principal Component Analysis

作　　者：李明珠[1] 宋平顺 赵建邦 

机构地区：[1]甘肃省兰州大学药学院,兰州730000 [2]甘肃省药品检验研究院,甘肃省中药品质与安全评价工程技术研究中心,兰州730000

出　　处：《中国实验方剂学杂志》2015年第8期81-85,共5页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的：建立HPLC测定烈香杜鹃中芦丁、金丝桃苷、槲皮苷、槲皮素、山柰酚和异鼠李素6种黄酮成分的方法,同时应用主成分分析评价其质量。方法：样品经75%甲醇超声,采用C18色谱柱（4.6 mm×250 mm,5μm）,乙腈-0.2%磷酸水为流动相梯度洗脱,流速1.0 m L·min^-1,检测波长365 nm。结果：芦丁,金丝桃苷,槲皮苷,槲皮素,山柰酚,异鼠李素在0.009 7-0.194 9,0.007 1-0.141 6,0.188 0-3.752,0.027 1-0.434 0,0.010 8-0.216 0,0.005 8-0.116 6μg线性范围良好,加样回收率均在100%-105%,RSD都〈3%。结论：HPLC操作简便、重复性好、结果准确。根据不同化学指标,采用主成分分析可以进行烈香杜鹃质量评价。Objective： To establish an HPLC method for the determination of rutin, hyperoside, quercitrin, quercetin, kaempferol and isorhamnetin in Rhododendron anthopogonoides, while using principal component analysis to evaluate its quality. Method： Samples were extracted with 75% methanol by ultrasound. The separation was performed on a C18 column （4.6 mm×250 mm,5μm） with acetonitrile-0.2% HaPO4 as mobile phase in a gradient elution. The flow rate was 1.0 mL ·min^-1, the wavelength was set at 365 nm. Result： Rutin, hyperoside, quercitrin, quercetin, kaempferol, and isorhamnetin have good linearity in the range of 0. 009- 0.1949, 0.007 1-0.141 6, 0.0.188 0-0.375 2, 0.027 1-0.434 0, 0.010 8-0.216 0, 0.005 8-0.116 6 μg, respectively. The recoveries were 100%-105% , RSD were 〈 3%. Conclusion： The established HPLC method was simple, accurate, reproducible, and reliable. Depending on the chemical indicators, using principal component analysis can be carried out quality assessment for R. anthopogonoides.

关 键 词：烈香杜鹃 主成分分析 芦丁 金丝桃苷 槲皮苷 槲皮素 山柰酚 异鼠李素 

分 类 号：R284.1[医药卫生—中药学]