温度对铁皮石斛组培苗质量的影响  

Influence of Temperature on Quality of Dendrobii Officinalis Caulis Tissue Culture Seedlings

作　　者：刘雪兰[1] 孙长生[1] 朱虹[1] 李利霞[1] 李婷婷[1] 龙祥友[1] 

机构地区：[1]贵阳药用植物园,贵阳550002

出　　处：《中国实验方剂学杂志》2015年第8期89-92,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵阳市科技计划项目[筑科合同(2012401)社-1号];贵阳市高技术产业发展专项(2012)

摘　　要：目的：考察温度对铁皮石斛组培苗质量的影响,为该药材的人工栽培提供参考。方法：将增殖阶段和生根阶段的铁皮石斛组培苗在湿度、光照强度、光照时间可控的环境条件下,设置温度分别为15,20,25,30,35℃共5个梯度进行实验,观察组培苗生长状况,利用Pearson相关性分析增殖阶段温度与苗株数、株高,生根阶段温度与株高、茎粗、叶片数、根数、根长的相关性,并对每个温度间生长指标差异性进行方差分析。结果：25-30℃的组培苗质量较好,表现出良好的生长和繁殖能力;Pearson相关性分析显示在增殖阶段温度跟苗株数和株高在0.01水平上显著相关;在生根阶段温度跟株高、叶片数、根数在0.01水平上显著相关。LSD多重比较显示在增殖阶段苗株数、株高的差异性明显;生根阶段叶片数、根数、株高差异性明显。结论：温度是影响铁皮石斛组培苗质量的关键因子,最适温度25℃。Objective： To investigate influence of temperature on quality of Dendrobii Officinalis Caulis tissue culture seedlings and provide a reference for artificial cultivation of this medicinal herb. Method： Dendrobii Officinalis Caulis tissue culture seedlings at muhiplicative and rooting stage were setted in the environment of humidity, light intensity and illumination time controlled, setting temperature were 15, 20, 25, 30, 35 ℃, growth conditions of tissue culture seedlings were observed. Pearson correlation was analyzed, variance analysis was adopted to analyze growth index difference among each temperature. Result： Tissue culture seedlings＇s quality was good at 25-30 ℃ and they showed good growth and reproductive capacity. Pearson correlation analysis showed that temperature with seedling number and plant height were significantly correlated at 0.01 level in proliferation stage; temperature with plant height, leaf number and root number were significantly correlated at 0.01 level in rooting stage. LSD multiple comparison showed that difference of seedling number and plant height were obvious in proliferation stage, difference of leaf number, root number and plant height were obvious in rooting stage. Conclusion： Temperature is the key factor for quality of Dendrobii Officinalis Caulis tissue culture seedlings and the most suitable temperature is 25 ℃.

关 键 词：铁皮石斛 组培苗 温度 质量指标 

分 类 号：S723.13[农业科学—林木遗传育种] R282.2[农业科学—林学]