高血压肝火亢盛证动物模型相关指标的研究  

Research of Relative indexes of Animal Models of Hypertension with Liver Fire Hyperactivity Syndrome

作　　者：薛晓兴 李玉波[2] 廉洪建 李君玲[1] 阎晶璐[1] 马雪玲[1] 王伟明[1] 王伟[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029 [2]中国中医科学院中医基础理论研究所,北京100700

出　　处：《中国实验方剂学杂志》2015年第8期97-101,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)项目(2011CB505106);北京中医药大学自主选题项目(2014-JYBZZ-XS-026)

摘　　要：目的：前期研究发现自发性高血压大鼠（SHR）14-18周龄表现为稳定肝火亢盛证,现从宏观表征及行为学,微观理化指标,方剂反证验证,初步研究高血压肝火亢盛证生物学机制。方法：14周龄SHR 55只经证候属性判别,筛选出肝火亢盛证SHR,根据收缩压差异用拉丁方分组法将其分为：模型组、三草降压汤组、四逆散组和硝苯地平组,正常血压大鼠（WKY）为正常组。动态采集大鼠宏观表征、行为学指标和收缩压;酶联免疫吸附试验（ELISA）法检测大鼠给药4,6周时血清5-羟色胺（5-HT）,血管紧张素Ⅱ（ANGⅡ）,去甲肾上腺素（NE）含量。结果：与正常组相比模型组14-20周龄血压值和易激惹程度升高（P〈0.05）,旋转耐受时间减少,且血清5-HT,ANGⅡ,NE升高。与模型组相比三草降压汤组和硝苯地平组给药4,6周收缩压降低（P〈0.05）,四逆散组收缩压先升高后降低,不稳定;三草降压汤组14-18周龄肝火亢盛证症状包括旋转耐受时间,易激惹程度和宏观表征有较明显的改善,四逆散组改善不明显,三草降压汤组血清5-HT,ANGⅡ,NE降低。结论：14-18周龄是SHR稳定的肝火亢盛证期,5-HT,ANGⅡ,NE升高可作为高血压肝火亢盛证客观指标。Objective： To investigate the biological basis of hypertension with hyperactivity of liver fire of macro characterization and behavioral tests, micro physicochemical indexes, disproof prescriptions. Method： Fifty-five SHR rats with hyperactivity of liver fire were divided into model group, Sancao Jiangya decoction （SCD） group, Sini San decoction （SND） group and nifedipine group according to Latin square method, Wistar-Kyoto （WKY） rats as control group. The macro characterization, behavioral indexes and the systolic blood pressure （SBP） were collected. Enzyme-linked immunosorbent assay （ELISA） was used to detect the serum concentration of 5-hydroxytryptamine （5-HT） , angiotensin Ⅱ （ ANG Ⅱ ）, norepinephrine （ NE ） after the rats were administrated for 4 and 6 weeks. Result： Compared with control group, the SBP and irritable degree increased, revolving tolerance time decreased, and serum 5-HT, ANG Ⅱ , NE level was higher in model group. Compared with model, the SBP in SCD group and nifedipine group reduced after treated for 4, 6 weeks, （18, 20 weeks of age） , the SBP in SND group was stable and increased in the 16 weeks, and then decreased. The symptoms of liver fire hyperactivity improved, and it was not obvious in SND group, and serum 5-HT, ANG Ⅱ , NE level was lower in SCD group compared with model group. Conclusion： Fourteen-eighteen weeks is the stable time window of hyperactivity of liver fire. SHR, 5-HT, ANG Ⅱ , NE may be used as the objective index of hypertension of hyperactivity of liver fire syndrome.

关 键 词：自发性高血压大鼠 肝火亢盛证 生物学机制. 

分 类 号：R285.5[医药卫生—中药学]