补肾化痰益智法对APP/PS1双转基因小鼠学习记忆及抗氧化能力的影响  

Effect of Replenishing Kidney-essence and Removing Phlegm on Ability of Learning and Memory and Antioxidant in APP/PS1 Double Transgenic Mice

作　　者：许湘[1] 石和元[1] 孔明望[1] 丁莉[1] 潘怡宏[1] 王平[1] 

机构地区：[1]湖北中医药大学老年医学研究所,湖北省老年病中药新产品协同创新中心,武汉430065

出　　处：《中国实验方剂学杂志》2015年第8期102-105,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金重点项目(81130064);湖北省首届医学领军人才专项(5406-0063)

摘　　要：目的:观察补肾化痰益智法对APP/PS1双转基因小鼠学习记忆及抗氧化能力的影响并探讨其可能机制。方法:随机将APP/PS1双转基因小鼠分为模型组、西药多奈哌齐组(6.5×10-4g·kg-1·d-1)、补肾化痰益智方高、低剂量组(15.6,7.8 g·kg-1·d-1),每组10只。空白组为10只同月龄相同遗传背景C57BL/6J小鼠。从3月龄开始对小鼠分别实施灌胃治疗,每日1次。空白组、模型组均给予等容积的生理盐水灌胃。连续灌胃3个月后,进行跳台实验及穿梭箱实验评定各组小鼠的学习记忆能力;采用比色法检测小鼠脑组织超氧化物歧化酶(SOD)和丙二醛(MDA)的含量。结果:跳台实验结果显示,与模型组比较,各组小鼠的潜伏期明显延长和出错次数明显减少(P<0.01);穿梭箱实验结果显示,与模型组比较,各组小鼠的主动回避次数明显增加(P<0.01,P<0.05),主动回避潜伏期、被动回避潜伏期明显缩短(P<0.01);西药组和补肾化痰益智方高、低剂量组小鼠脑组织中SOD活性较模型组显著升高(P<0.01),MDA含量明显降低(P<0.01)。结论:补肾化痰益智方对APP/PS1双转基因小鼠的学习记忆具有一定的改善作用,且可能与其抗氧化作用有关。Objective： To investigate the effect of replenishing kidney-essence and removing phlegm on the ability of learning and memory and antioxidant in APP/PS1 double transgenic mice. Method： APP/PS1 double mice were randomly divided into model group, western medicine group （6.5×10^-4g·kg^-1·d^-1）, Bushen Huatan Yizhi recipe （BSHTYZR） high dose group （15.6,7.8 g·kg^-1·d^-1 ）, low dose group （7.8 g·kg^-1·d^-1） , 10 in each groups. Blank group mice （C57BL/6J） were 10 months of age with the same genetic background mice. After intragastrical administration for 3 months, the ability of learning and memory was assessed by the step down avoidance test and shuttle box test; colorimetric assay was used to measure mouse brain tissue superoxide dismutase （SOD） and malondialdehyde （MDA） content. Result： Step down avoidance test results showed that compared with model group, each group of mice significantly prolonged the incubation period and the number of errors decreased significantly （P 〈 0.01 ） ; shuttle box test results showed that compared with model group, the number of active avoidance in treatment groups increased significantly （ P 〈 0.01, P 〈 0.05 ） , and active avoidance incubation period, the passive avoidance period significantly shorten （P 〈 0.01 ） ; shuttle box test results showed that compared with the model group, in the rest of the administration group the small active avoidance significantly increased the number of rats （ P 〈 0.01, P 〈 0.05 ） , while the active avoidance latency and passive avoidance latency was shorter （P 〈 0.01 ） ; in addition, in BSHTYZR high dose group and low dose group SOD activity of brain tissue increased significantly compared with the model group （P 〈 0.01 ）, MDA was significantly lower than the model group （P 〈0.01 ）. Conclusion： BSHTYZR has a certain improvement on APP/ PS1 double transgenic mice learning and memory, and may be related to the antioxidant effects.

关 键 词：补肾化痰益智方 阿尔茨海默病 跳台实验 穿梭箱实验 抗氧化 

分 类 号：R285.5[医药卫生—中药学]