紫金莲醇提物2种萃取部位镇痛作用机制探讨  

Study on Analgesic Mechanisms in CNS with Extraction Part of National Medicine Ceratostigma willmottianum

作　　者：卢启振 黄聪[1] 邓炜[1] 

机构地区：[1]贵阳中医学院,贵阳550002

出　　处：《中国实验方剂学杂志》2015年第8期141-144,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省科技厅和贵阳中医学院联合资金项目(黔科合中药字[2011]LKZ7033号)

摘　　要：目的:探讨紫金莲醇提物2种萃取部位对甲醛致痛大鼠模型的镇痛作用及机制。方法:56只SD大鼠随机分6组,每组8只,分别设为正常组,模型组,紫金莲醇提物乙酸乙酯部位高(乙高组30 mg·kg-1)、低(乙低组15 mg·kg-1)剂量组,紫金莲醇提物正丁醇部位高(正高组,15 mg·kg-1)、低(正低组,8 mg·kg-1)剂量组。各给药组按剂量ig大鼠,给药7 d,每日1次。第7天末次给药40 min后,除正常组外,其余各组大鼠左后足跖sc 10%甲醛溶液50μL/只,建立大鼠甲醛致痛模型。观察紫金莲2种活性部位对甲醛致痛模型大鼠痛行为的影响,生化法检测大鼠血清谷氨酸和Ca2+含量,免疫组化法检测大鼠脑和脊髓c-fos阳性细胞表达和P物质(SP)含量。结果:与正常组比较,模型组大鼠表现出了典型的两时相痛行为学反应,且在脑和脊髓中检测到了大量的c-fos阳性细胞,P物质(SP),升高血清谷氨酸及Ca2+含量有显著性差异(P<0.05),表示造模成功。与模型组比较,紫金莲乙低组(15 mg·kg-1)和正低组(8 mg·kg-1),对大鼠第一时相伤害反应疼痛有显著的抑制作用(P<0.05),使大鼠血清谷氨酸和脑c-fos蛋白表达显著下降(P<0.05),血清Ca2+含量显著升高(P<0.05);紫金莲醇提物乙高组(30 mg·kg-1)和正高组(15 mg·kg-1)显著降低大鼠脊髓c-fos蛋白表达(P<0.05);紫金莲醇提物乙高、乙低组和紫金莲醇提物正高、正低组都能显著降低SP在大鼠脑和脊髓中的表达(P<0.05)。结论:紫金莲醇提物2种萃取部位对大鼠甲醛致痛模型均有镇痛作用,其镇痛机制可能与抑制体内谷氨酸的升高,抑制细胞内Ca2+浓度升高及降低c-fos,SP在中枢神经系统内的表达有关。willmottianum （CW） Objective： To study the analgesic mechanisms of two extraction part of Ceratostigma in rat pain model induced by formaldehyde. Method： Fifty-six SD rats were randomly divided into 6 groups with 8 in each group, including normal group, model group, CW alcohol extract＇s ethyl acetate part group （30, 15 mg·kg^-1）, n-butanol part group （15.8 mg·kg^-1 ）. Each group was given corresponding drugs by intragastric injection, respectively, once a day for 7 days. At the 7th day, the animals were fasted for 12 hours, and ig 40 min later, except normal group, the other groups were given subcutaneous injection of 10% formaldehyde solution 50 μL for establishing the pain models. The impact of CW active site was observed and serum glutamate and Ca^2＋ were detected with biochemical kit, and the expression of c-fos and substance P （SP） was assayed with immunohistochemeistry （IHC）. Result： Compared with normal group, model group showed a typical pain behavior, amounts of c-fos and SP were detected in the brain and spinal cord, and raised serum glutamic acid concentration, Ca^2＋ content with significant difference （P 〈 0.05）, meaning that the pain model of rats induced by formaldehyde was successful. Compared with model group, the low dose of ethyl acetate part of CW alcohol extract （15 mg ·kg^-1） and n-butanol part （8 mg ·kg^-1） can obvious inhibition to the first phase damage response （P 〈 0. 05）, make serum glutamic content, brain c-fos protein expression decreased significantly （ P 〈 0. 05 ） , serum Ca^2＋ increased significantly （ P 〈 O. 05 ） ; the high dose of two parts decreased the expression of c-fos in spinal dorsal cord significantly （P 〈 0. 05 ） ; both of high and low dose of CW alcohol extract＇s ethyl acetate and n-butanol part decreased the expression of SP significantly （P 〈 0. 05 ） in central nervous system （ CNS）. Conclusion： Two extraction parts of CW alcohol extract have exact analgesic action in rat pain m

关 键 词：紫金莲 萃取部位 镇痛 机制 

分 类 号：R285.5[医药卫生—中药学]