一次性吸管萃取-气相色谱/质谱法测定白菜中有机磷农药残留  

Disposable Pipette Extraction for the Analysis of Organophosphate Pesticides in Cabbage Using Gas Chromatography/Mass Spectrometry

作　　者：宋宁慧[1] 张静[1] 荣维广[2] 

机构地区：[1]环境保护部南京环境科学研究所,江苏南京210042 [2]江苏省疾病预防控制中心,江苏南京210009

出　　处：《分析科学学报》2015年第2期213-217,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.41101307);江苏省"十二.五"科教兴卫工程(No.ZX201109)

摘　　要：建立了一次性吸管萃取（Disposable Pipette Extraction,DPE）-气相色谱/质谱联用法测定白菜中有机磷农药残留的分析方法。利用DPE所固有的快速混合功能使得待测农药从样品基质中快速而有效地分离,并试验了最小体积溶剂洗脱方式,大大减少了溶剂的使用量,同时省去了溶剂蒸发的步骤,节约了成本提高了效率。该方法前处理过程是一种简便、经济、高效的方法。最后采用气相色谱/质谱选择离子模式和外标法对待测农药进行定性和定量。结果表明：非极性有机磷农药（logP值≥2）的回收率在80.5%-105.3%之间,相对标准偏差小于13%,所有组分检出限在5.9μg/kg以下,定量限在17.7μg/kg以下。适用于蔬菜中有机磷类农药残留的快速检测。A rapid analytical method for determination of organophosphorus pesticides in cabbage was established based on disposable pipette extraction（DPE）and gas chromatography-mass spectrometryselective ion monitoring（GC/MS-SIM）.The intrinsic rapid mixing capabilities of DPE result in fast and efficient extractions,and eluates are concentrated by using minimal elution solvent volumes rather than solvent evaporation methods.The pretreatment process is easy,simple,economic and efficient.The organophosphorus pesticide residues were analyzed by GC/MS-SIM and quantified with external standards.The results showed average recoveries between 80.5% and 105.3%for nonpolar and slightly polar pesticides（logP≥2）,with relative standard deviations（RSD）〈13%.The limits of detection of the method were below 5.9μg/kg and the limits of quantitation were below 17.7μg/kg.The method is simple and rapid and applicable to determine organophosphorus pesticide residues in vegetables.

关 键 词：有机磷农药 一次性吸管萃取 气相色谱/质谱联用法 农药残留 白菜 

分 类 号：O657.63[理学—分析化学]