高效液相色谱法同时测定款冬花中四种酚酸  

Simultaneous Determination of Four Phenolic Acids in Farfarae flos by High Performance Liquid Chromatography

作　　者：冯海燕[1] 杨晓辉[1] 岳红坤[1] 陆敏[1] 李彦楠[1] 

机构地区：[1]石家庄学院化工学院,河北石家庄050035

出　　处：《分析科学学报》2015年第2期281-284,共4页Journal of Analytical Science

基　　金：石家庄学院科研项目(13YB002)

摘　　要：建立了同时测定款冬花中没食子酸、绿原酸、咖啡酸和阿魏酸的高效液相色谱方法。采用Sepax Gp-C18色谱柱（250×4.6mm,5μm）,以甲醇-0.5%乙酸溶液为流动相梯度洗脱,流速1.0mL/min,柱温30℃,检测波长280nm,20min内4种酚酸可实现基线分离。结果表明,各组分在一定浓度范围内呈良好线性关系,相关系数均大于0.9990,检测限为0.05-0.14mg/L,平均回收率在92.3%-102.0%之间,相对标准偏差为0.27%-2.70%,且不同来源的款冬花中4种酚酸的含量有明显差异。该方法简便、快速、准确、重复性好,可作为款冬花药材质量控制的方法。A high performance liquid chromatographic method was developed for simultaneous determination of gallic acid,chlorogenic acid,caffeic acid and ferulic acid in Farfaraeflos.The separation was performed on a Sepax Gp-C18column（250×4.6mm,5μm）by gradient elution using 0.5%acetic acid and methanol as the mobile phase at a flow rate of 1.0mL/min.The column temperature was maintained at 30℃,and the detection wavelength was 280 nm.Under these conditions,four phenolic acids could be baseline separated within 20 min.Good linearity was observed between the concentrations and chromatographic peak areas with correlation coefficients over 0.9990.Limits of detection and average recoveries of the tested compounds varied from 0.05 to 0.14mg/L and 92.3%to 102.0% with RSD of 0.27%-2.70%.The results indicated that four analytes in Farfarae flos from different origins showed obvious differences in the content.The established method is simple,rapid and accurate with a good reproducibility,and can be used for quality control of Farfarae flos.

关 键 词：高效液相色谱 款冬花 酚酸 

分 类 号：O657.72[理学—分析化学]