双波长双指示剂催化动力学光度法测定痕量Fe(Ⅲ)  

Dual-Wavelength Dual-Indicator Catalytic Kinetic Spectrophotometry for Determination of Trace Fe(Ⅲ)

作　　者：侯法菊[1] 陈玉静[1] 苗延虹[2] 徐慧[1] 

机构地区：[1]鲁东大学化学与材料科学学院,山东烟台264025 [2]山东农业大学化学与材料科学学院,山东泰安271000

出　　处：《分析科学学报》2015年第2期294-296,共3页Journal of Analytical Science

基　　金：国家自然科学基金(No.21104030);山东省优秀中青年科学家科研奖励基金(No.BS2009SW040)

摘　　要：研究了在HCl介质中,Fe（Ⅲ）催化H2O2氧化甲基橙和亚甲基蓝褪色的指示反应。通过测量510nm和660nm波长处催化和非催化体系吸光度的变化,基于Fe（Ⅲ）的浓度与两波长处吸光度差值的加和呈线性关系,建立了双波长双指示剂催化动力学光度法测定Fe（Ⅲ）含量的新方法。方法的线性范围为0.008-0.24μg/mL,用该法测定菠菜、紫菜、蛋黄中Fe（Ⅲ）含量,相对标准偏差为1.0%-4.6%,加标回收率为98.9%-104.6%。The oxidization fading of methy1 ene blue and methyl orange catalyzed by trace iron in HC1 medium was studied.And,a new dual-wave1 ength and dual-indicator catalytic kinetic spectrophotometric method was established for the determination of trace iron.The absorbance of catalytic and non-catalytic systems was measured at 510 nm and 660 nm,respectively.The concentration of Fe（Ⅲ）showed linear relationship with the difference between absorbance at two wavelengths.The linear range of method was0.008-0.24μg/mL.The method could be applied to the determination of trace Fe（Ⅲ）in samples（such as spinach,laver and yolk）without pre-separation,giving the relative standard deviations of 1.0%-4.6% and the recoveries of 98.9%-104.6%.

关 键 词：Fe(Ⅱ) 双波长 双指示剂 催化光度法 

分 类 号：O657.32[理学—分析化学]