正交设计法优选跌打促愈片的水提工艺  

Extraction technology to optimize diedacuyu tablets by orthogonal design

作　　者：余辉[1] 陈卫红[1] 蒋孟良[2] 金晓飞[2] 银小娟 张春林[3] 

机构地区：[1]湖南中医药大学第二附属医院,湖南长沙410005 [2]湖南中医药大学,湖南长沙410208 [3]河南省洛阳正骨医院,河南洛阳471013

出　　处：《中国医院药学杂志》2015年第7期605-608,共4页Chinese Journal of Hospital Pharmacy

基　　金：湖南省科技厅资助项目(编号:2013SK3104);国家中医药管理局重点学科资助

摘　　要：目的：优选跌打促愈片的提取工艺。方法：以丹酚酸B含量、川续断皂苷Ⅵ含量和水溶性浸出物为评价指标,采用正交设计法优选跌打促愈片的提取工艺。结果：丹酚酸B在0.37~2.96μg之间,线性关系良好,其回归方程为：Y=407.34 X-17.824,r=0.999 8;川续断皂苷Ⅵ在0.8~6.4μg之间,线性关系良好,其回归方程为：Y=255.94 X＋5.095 1,r=0.999 5。跌打促愈片的最佳提取工艺为：浸泡2 h,加水煎煮2次,第1次加10倍量水提取1.5 h,第2次加8倍量水提取1.0h。结论：优选出的工艺可行,重复性好,可用于该制剂的提取。OBJECTIVE To select the best extraction process of Diedacuyu Tablets.METHODS Taking the indexes of salvia acid B,asperosaponin Ⅵ content and extract water-soluble,the extraction process of Diedacuyu Tablets were optimized with orthogonal design test.RESULTS The salvia acid B showed a good linear relationship in the range of 0.37-2.96μg,the regression equation was Y=407.34 X-17.824,r=0.999 8.The asperosaponin Ⅵ showed a good linear relationship in the range of 0.37-2.96μg,the regression equation was Y=255.94 X＋5.095 1,r=0.999 5.The best extraction process were soaking for 2 h,boiling water for 2 times,first 10 times the amount of water extraction1.5 h,second 8 times water extraction of 1.0h.CONCLUSION The process is feasible and has good repeatability.It can be used for extraction of this preparation.

关 键 词：跌打促愈片 正交设计 提取工艺 丹酚酸B 川续断皂苷Ⅵ 

分 类 号：R283.5[医药卫生—中药学]