拉曼光谱法快速测定盐酸异丙嗪注射液的含量  

Rapid content determination of promethazine hydrochlorine injection by Raman spectroscopy

作　　者：赖源发[1] 胡佳[1] 林丽丽[1] 张启国 

机构地区：[1]厦门华厦职业学院食品药品安全重点实验室,福建厦门361024 [2]厦门市药品检定所,福建厦门361000

出　　处：《中国医院药学杂志》2015年第7期612-615,共4页Chinese Journal of Hospital Pharmacy

基　　金：福建省教育厅科技类项目(编号:JA13451)

摘　　要：目的：建立拉曼光谱法快速测定盐酸异丙嗪注射液的含量。方法：采用智能激光拉曼光谱仪扫描样品,激发波长780nm,激光强度100 MW,光圈25μm,光栅400 gr·mm-1,分辨率2.0cm-1,曝光时间50 s,曝光次数2次,扫描范围100~3 200cm-1。结果：盐酸异丙嗪在20~200 mg·ml-1范围内与拉曼定量峰面积呈良好的线性关系（r=0.998 2）,平均加标回收率为98.13%,RSD为1.05%（n=9）。结论：本方法快速、简单、可靠,可用于盐酸异丙嗪注射液的含量测定。OBJECTIVE To establish a rapid raman spectroscopy method for content determination of promethazine hydrochloridne injection.METHODS Smart laser raman spectrometer was used for determination.The excitation wavelength was780 nm,laser power 100 MW,aperture 25μm,grating400 gr·mm-1,resolution2.0cm-1,exposure time 50 s,exposure times2,and the scanning range 100- 3 200 cm-1.RESULTS The calibration curve was linear over the range of 20-200 mg·ml-1（r=0.998 2）,and the average recovery was 98.13% with RSD of 1.05%（n=9）.CONCLUSION The method is convenient and rapid.It is able to be used in the rapid determination of injection.

关 键 词：拉曼光谱 盐酸异丙嗪 注射液 快速测定 

分 类 号：R927.2[医药卫生—药学]