GC法同时测烧伤软膏中甲基正壬酮与冰片  

Determination of methyl nonyl ketone and borneol from Burn Ointment by GC

作　　者：张建春[1,2] 安宝文 何家田[3] 王俊丽[3] 盖静[3] 

机构地区：[1]中国人民解放军第一医院,甘肃兰州730030 [2]兰州大学药学院,甘肃兰州730000 [3]兰州军区联勤部药品仪器检验所,甘肃兰州730050

出　　处：《中成药》2015年第4期782-785,共4页Chinese Traditional Patent Medicine

基　　金：军队医药卫生科研基金课题资助(CLZ2JB22)

摘　　要：目的建立毛细管气相色谱法同时测定烧伤软膏中甲基正壬酮和冰片。方法 Agilent HP-5毛细管色谱柱（30 m×0.32 mm,0.25μm）,FID检测器,柱温采取程序升温,进样口温度220℃,检测器温度250℃,进样量1μL,不分流,载气为氮气,柱体积流量1.0 m L/min,空气体积流量450 m L/min,氢气体积流量40 m L/min。结果 2组分的分离度和线性关系良好,甲基正壬酮、冰片线性范围分别为4.652-116.3μg/m L（r=0.999 9）、0.2098-5.246 mg/m L（r=0.999 8）,平均回收率分别为98.1%（RSD=1.7%）、98.3%（RSD=1.8%）。结论本法操作简便、结果准确、重复性好,可用于烧伤软膏的质量控制。AIM To establish a method for determining methyl nonyl ketone and borneol in Burn Ointment.METHODS The methyl nonyl ketone and borneol were determined by gas chromatography with Agilent HP-5capillary chromatographic column（ 30 m × 0. 32 mm,0. 25 μm）. The column was in programming temperature mode,220 ℃ injector temperature,250 ℃ FID temperature,1μL injector volume were adopted without split ratio.Nitrogen was used as carrier gas with 1. 0 m L / min volume flow of column,400 m L / min air and 40 m L / min hydrogen. RESULTS The linear ranges were 4. 652- 116. 3 μg / m L（ r = 0. 999 9） for methyl nonyl ketone,0. 209 8- 5. 246 mg / m L（ r = 0. 999 8） for borneol,respectively. The corresponding average recovery rates were98. 1%（ RSD = 1. 7%） and 98. 3%（ RSD = 1. 8%）,respectively. CONCLUSION The established gas chromatography presents simplicity,accuracy,high sensitive and good reproducibility,it can be used for quality control of Burn Ointment.

关 键 词：烧伤软膏 气相色谱法 冰片 甲基正壬酮 

分 类 号：R927.2[医药卫生—药学]