抗禽流感抗体在广东省人群中的分布研究  

Sero-prevalence of antibodies against avian influenza virus among people in poultry occupations in Guangdong province

作　　者：武婕[1] 倪汉忠[1] 邹丽容[1] 宋颖超[1] 李来庆[1] 亓文宝[1] 康敏[1] 梁丽君[1] 张欣[1] 钟豪杰[1] 黄国锋[1] 林锦炎[1] 廖明[1] 柯昌文[1] 

机构地区：[1]广东省疾病预防控制中心,广州511430

出　　处：《中华微生物学和免疫学杂志》2015年第2期94-98,共5页Chinese Journal of Microbiology and Immunology

基　　金：广东省科技计划项目（20138020307006）;广州市科技计划项目（2013J4200020,2014J4100091）

摘　　要：目的：检测广东省禽接触职业人群携带抗H5、H6、H7和H9禽流感病毒（ avian influ-enza virus， AIV）抗体阳性率，评估AIV的隐性感染率及传播活性的变化。方法以广东省10个地市的1066名禽接触职业人员血清和205名非禽接触职业人群血清为研究对象，以从广东省外环境或者禽中分离的灭活病毒为检测抗原，用血凝抑制法检测广东省人群携带抗H5、H6、H7和H9抗体滴度。结果2013年广东省禽接触职业人群携带抗H5、H6、H7和H9的抗体阳性率分别是0．44％、0％、0．30％和0．30％；2014年广东省禽接触职业人群携带抗 H5、H6、H7和H9的抗体阳性率分别是1．08％、0．0％、0．0％和0．27％；而对照组只有2013年非禽接触职业人群的抗H9抗体阳性率是0．95％，其余3种AIV的抗体都没有阳性人群。禽接触职业人群和非禽接触职业人群携带AIV抗体阳性率差异没有统计学意义，但是职业人群的抗体几何平均滴度高于非职业人群。结论广东省人群普遍不携带抗AIV抗体，AIV隐性感染率低，尚不具备大规模向人间传播的能力，但是与禽接触仍是人群感染AIV的主要风险因子，应长期开展对禽接触职业人群的AIV抗体监测，及时预防和预警新型AIV由禽向人的传播。Objective To detect the positive rates of antibodies against avian influenza virus （AIV） subtypes H5, H6, H7 and H9 among people in poultry occupations in Guangdong province and to analyze the transmission of various subtypes of AIV from poultry to human contacts for the prevention and control of novel AIV infection in human beings.Methods Serum specimens were collected from 1066 peo-ple in poultry occupations （ occupational group） and 205 people not in poultry occupations （ non-occupational group） in 10 cities of Guangdong province.The inactivated AIV strains, isolated from poultry or environment of Guangdong province, were used as antigens to detect antibodies against AIV subtypes H5, H6, H7 and H9 by using the hemagglutination inhibition （ HI） assay.Results The positive rates of antibodies against AIV subtypes H5, H6, H7 and H9 carried by people from the occupational group were respectively 0.44%, 0%, 0.30%and 0.30%in 2013 and 1.08%, 0.0%, 0.0%and 0.27%in 2014.Only the anti-H9 anti-bodies were detected in serum samples collected form people in the non-occupational group in 2013 with a positive rate of 0.95%.No significant differences with the positive rates of anti-AIV antibodies were found between the occupational group and the non-occupational group.However, the geometric mean titer （ GMT） of anti-AVI antibodies in people from the occupational group was higher than that of the non-occupational group.Conclusion Although a grand spread of AIV from avian to human is not likely to happen yet, con-tacting with poultry is the risk factor for AIV infection in Guangdong population.A long-term surveillance of anti-AIV antibodies in serum should be strengthened among people in poultry occupations for the timely pre-vention and control of novel AIV outbreak.

关 键 词：禽流感病毒 职业人群 抗体检测 

分 类 号：R373[医药卫生—病原生物学]