Ghrelin基因在多囊卵巢综合征患者卵巢颗粒细胞中的表达  

Expression of Ghrelin gene in ovarian granulosa cells of patients with polycystic ovarian syndrome

作　　者：王炼炼[1] 李聪[2] 杨曦[1] 孟江萍[1] 幸贵邦[1] 

机构地区：[1]重庆医科大学附属第一医院生殖健康与不孕专科,重庆400016 [2]重庆医科大学附属第一医院妇产科,重庆400016

出　　处：《重庆医科大学学报》2015年第2期239-242,共4页Journal of Chongqing Medical University

基　　金：国家自然基金青年基金资助项目(编号:81100399);重庆市科委资助项目(编号:CSTC2011jj A0111)

摘　　要：目的:探讨Ghrelin基因在卵巢颗粒细胞中的表达及其与多囊卵巢综合征(polycystic ovarian syndrome,PCOS)的关系。方法:选择47例在重庆医科大学辅助生育中心接受体外受精-胚胎移植治疗的不孕妇女,其中PCOS组11例,对照(Non-PCOS)组36例,采用real-time PCR及免疫印迹法分别测定2组妇女卵巢颗粒细胞中Ghrelin m RNA和蛋白的表达水平。结果:PCOS妇女颗粒细胞中Ghrelin m RNA和蛋白的表达均低于Non-PCOS妇女(0.926±0.206 vs.1.137±0.105,t=2.73,P=0.015;0.647±0.052 vs.0.937±0.038,t=7.82,P=0.001)。结论:PCOS妇女卵巢颗粒细胞的Ghrelin m RNA和蛋白表达水平明显低于Non-PCOS妇女,可能与卵泡发育障碍相关。Objective：To determine the expression of Ghrelin gene in ovarian granulosa cells（GCs）of patients with polycystic ovarian syndrome（PCOS）and to discuss the relationship between them. Methods：GCs were obtained at the time of oocyte retrieval from a total of 47 women undergoing in vitro fertilization and embryo transfer（IVF-ET）treatment due to male factors or tubal factors. Eleven PCOS women were taken as PCOS group and 36 non-PCOS women as control group. The expression of Ghrelin m RNA was measured by real-time PCR and Western blot was used to detect the expression of Ghrelin protein in GCs. Results：The expression of Ghrelin m RNA in GCs was significantly lower in PCOS group than in control group（t=2.73,P=0.015）and the expression of Ghrelin protein in GCs was also significantly lower in PCOS group than in control group（t = 7. 82,P = 0. 001）. Conclusion ：The m RNA and protein expressions of Ghrelin in GCs are significantly lower in women with PCOS than in non-PCOS women,suggesting that Ghrelin may be involved in the disturbance of follicular development in PCOS.

关 键 词：GHRELIN基因 卵巢颗粒细胞 多囊卵巢综合征 

分 类 号：R711.75[医药卫生—妇产科学]