铜绿蝇幼虫在不同脏器组织源食物上生长发育变化  

Growth and development of lucilia cuprina larvae fed by different organ tissues

作　　者：王尧[1] 杜琳敏[2] 袁增琼[2] 李学博[2] 万立华[2] 

机构地区：[1]贵阳医学院法医学系,贵阳550004 [2]重庆医科大学基础医学院法医学教研室,重庆400016

出　　处：《重庆医科大学学报》2015年第2期285-288,共4页Journal of Chongqing Medical University

摘　　要：目的:研究铜绿蝇幼虫在不同组织源的食物上生长发育变化。方法:在26℃恒温、相对湿度70%条件下,用猪的肌肉组织、肝脏组织、脑组织、肌肉与脂肪的混合物(6︰4)分别饲养铜绿蝇幼虫。每隔12 h测体长和体质量,化蛹后测蛹的体长和体质量。统计幼虫及蛹的死亡率。推算各组幼虫发育总历期和成虫的性别比。结果:40%脂肪混合组与其他3组相比生长缓慢,达到最大个体的时间延迟24 h以上。肝脏组幼虫体长(15.02±1.39)mm和40%脂肪混合组体长(14.85±1.43)mm明显小于脑组织组(17.53±2.08)mm、肌肉组(16.82±2.15)mm(F=5.440,P=0.003);肝脏组幼虫体质量(72.8±14.2)mg和40%脂肪混合组体质量(70.4±15.4)mg均明显小于脑组织组(89.6±19.2)mg和肌肉组(85.9±16.7)mg(F=3.320,P=0.030)。40%脂肪混合组蛹体长及体重均明显小于脑组织组和肌肉组(F=7.890,P=0.001;F=9.280,P=0.001)。40%脂肪混合组幼虫和蛹的死亡率均明显高于脑组织组、肌肉组、肝脏组(F=3.470,P=0.040;F=4.720,P=0.035)。结论:铜绿蝇幼虫在40%脂肪混合食物上生长发育历期较长,肝脏组和40%脂肪混合组幼虫及蛹的体长和体质量较小。Objective：To research the growth and development of lucilia cuprina larvae fed by different organization sources. Methods：Under the condition of constant temperature（26 ℃）and relative humidity（70%）,muscle tissue,hepatic tissue,brain tissue,muscle and fat mixture（6∶4）were used to feed lucilia cuprina larvae. Length and weight of larvae and were measured at an interval of 12 h while length and weight of pupa were measured after eclosion. Development stages of larvae,mortality of larvae and pupa as well as sex ratio of adults were recorded. Results：Growth was retarded in 40% fat mixed group than in the other three groups,achieving maximum individual delay time of 24 h. Larvae body length of hepatic tissue group（15.02±1.39）mm and 40% fat mixed group（14.85±1.43）mm were statistically shorter than those of brain tissue group（17.53±2.08）mm and muscle tissue group（16.82±2.15）mm（F=5.440,P=0.003）. Larvae body weight of hepatic tissue group（72.8 ±14.2） mg and 40% fat mixed group（70.4 ±15.4） mg were statistically lighter than those of brain tissue group（89.6±19.2）mg and muscle tissue group（85.9±16.7）mg（F=3.320,P=0.030）. Pupa body weight and body length of 40% fat mixed group were statistically lighter and shorter than those of brain tissue group and muscle tissue group（F=7.890,P=0.001;F=9.280,P=0.001）. Larvae and pupa mortality of 40% fat mixed group were statistically higher than that in the other three groups（F=3.470,P=0.040;F=4.720,P=0.035）. Conclusion：Lucilia cuprina larvae fed by 40% fat mixed food have longer growth and development period. Body weight and body length of larvae and pupa are lighter and shorter in hepatic tissue group and 40% fat mixed group than in the other groups.

关 键 词：死后间隔时间 铜绿蝇幼虫 法医昆虫学 

分 类 号：R384.2[医药卫生—医学寄生虫学]