Effect of Dietary Lipid on the Growth, Fatty Acid Composition and Δ5 Fads Expression of Abalone(Haliotis discus hannai Ino) Hepatopancreas  

作　　者：LI Mingzhu MAI Kangsen AI Qinghui HE Gen XU Wei ZHANG Wenbing ZHANG Yanjiao ZHOU Huihui LIUFU Zhiguo 

机构地区：[1]The Key Laboratory of Aquaculture Nutrition and Feed of Ministry of Agriculture,The Key Laboratory of Mariculture of Ministry of Education, Ocean University of China [2]College of Agriculture, Ludong University

出　　处：《Journal of Ocean University of China》2015年第2期317-324,共8页中国海洋大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 30871930)

摘　　要：This study investigated the effect of dietary lipid on the growth, fatty acid composition and Δ5 fatty acyl desaturase genes(Fads) expression of juvenile abalone(Haliotis discus hannai Ino) hepatopancreas. Six purified diets were formulated to contain tripalmitin(TP), olive oil(OO, 72.87% 18:1n-9), grape seed oil(GO, 68.67% 18:2n-6), linseed oil(LO, 70.48% 18:3n-3), ARA oil(AO, 41.81% ARA) or EPA oil(EO, 44.09% EPA and 23.67% DAH). No significant difference in survival rate was observed among abalone fed with different diets. Weight gain rate(WGR) and daily growth rate of shell length(DGRSL) were significantly increased in abalone fed with diets containing OO, AO and EO, but decreased in abalone fed with LO diet(P < 0.05) in comparison with those fed with TP. High level of dietary 18:2n-6 resulted in higher content of n-6 polyunsaturated fatty acids(PUFAs) in abalone fed with GO than those fed with TP, OO, LO and EO(P < 0.05). n-3 PUFAs in abalone fed with LO was significantly higher than those in abalone fed with TP, OO, GO and AO(P < 0.05). The highest contents of 20:1n-9 and 22:1n-9 were observed in abalone fed with OO. The expression of Δ5 Fads in hepatopancreas of abalone was enhanced by high concentration of 18:3n-3 and suppressed by dietary LC-PUFAs; however it was not affected by dietary high concentration of 18:1n-9 or 18:2n-6. These results provided valuable information for understanding the synthesis of LC-PUFAs and nutritional regulation of Δ5 Fads expression in abalone.This study investigated the effect of dietary lipid on the growth, fatty acid composition and △5 fatty acyl desaturase genes （Fads） expression of juvenile abalone （Haliotis discus hannai Ino） hepatopancreas. Six purified diets were formulated to con- tain tripalmitin （TP）, olive oil （OO, 72.87% 18：1n-9）, grape seed oil （GO, 68.67% 18：2n-6）, linseed oil （LO, 70.48% 18：3n-3）, ARA oil （AO, 41.81% ARA） or EPA oil （EO, 44.09% EPA and 23.67% DAH）. No significant difference in survival rate was observed among abalone fed with different diets. Weight gain rate （WGR） and daily growth rate of shell length （DGRsL） were significantly increased in abalone fed with diets containing OO, AO and EO, but decreased in abalone fed with LO diet （P〈0.05） in comparison with those fed with TP. High level of dietary 18：2n-6 resulted in higher content of n-6 polyunsaturated fatty acids （PUFAs） in abalone fed with GO than those fed with TP, OO, LO and EO （P〈0.05）. n-3 PUFAs in abalone fed with LO was significantly higher than those in abalone fed with TP, OO, GO and AO （P〈0.05）. The highest contents of 20：1n-9 and 22：1n-9 were observed in abalone fed with OO. The expression of △5 Fads in hepatopancreas of abalone was enhanced by high concentration of 18：3n-3 and suppressed by dietary LC-PUFAs; however it was not affected by dietary high concentration of 18：1n-9 or 18：2n-6. These results provided valuable information for understanding the synthesis of LC-PUFAs and nutritional regulation of △5 Fads expression in abalone.

关 键 词：DESATURASE fatty acid gene expression  fish oil vegetable oil 

分 类 号：S968.3[农业科学—水产养殖]