Purification of a Diatom and Its Identification to Cylindrotheca closterium  

作　　者：WANG Song ZHANG Lin YANG Guanpin ZHU Baohua PAN Kehou 

机构地区：[1]Laboratory of Applied Microalgae Biology, Key Laboratory of Mariculture of Ministry of Education of China,Ocean University of China [2]College of Marine Life Sciences, Ocean University of China

出　　处：《Journal of Ocean University of China》2015年第2期357-361,共5页中国海洋大学学报（英文版）

基　　金：supported by the Major State Basic Research Development Program of China (973 Program) (2011CB200901);National Technical Supporting Project Foundation (2011BAD14B01);Energy Project from State Bureau of Oceanic Administration (Grant No. GHME2011SW03)

摘　　要：A diatom was purified with colony selection and continuous dilution methods. It was identified to Cylindrotheca closterium according to its morphological characteristics and rbc L and 18 s r RNA gene sequences. The alga was not sensitive to ampicillin and neomycin, but sensitive to chloramphenicol which inhibited its growth at concentrations ranging from 50 to 150 μg m L-1. The purified alga was easy to culture and its specific growth rate was 0.207 ± 0.002(d-1). It was resistant to pollution and could be harvested in an easy way. It was relatively high in lipid content(20.08% ± 0.67% of dry weight) and the combined amount of its 16:0 and 16:1(n-7), the most suitable resource of biodiesel, was as high as 64% of the total fatty acids, while the amount of polyunsaturated fatty acids reached 19.96%–20% of the total fatty acids. Thus the purified C. closterium can be cultured as a biodiesel producer or a nutrition supplement producer.A diatom was purified with colony selection and continuous dilution methods. It was identified to Cylindrotheca closte- rium according to its morphological characteristics and rbcL and 18s rRNA gene sequences. The alga was not sensitive to ampicillin and neomycin, but sensitive to chloramphenicol which inhibited its growth at concentrations ranging from 50 to 150 μgmL-1. The purified alga was easy to culture and its specific growth rate was 0.207±0.002 （d-1）. It was resistant to pollution and could be har- vested in an easy way. It was relatively high in lipid content （20.08%±0.67% of dry weight） and the combined amount of its 16：0 and 16：1 （n-7）, the most suitable resource of biodiesel, was as high as 64% of the total fatty acids, while the amount of polyunsatu- rated fatty acids reached 19.96%-20% of the total fatty acids. Thus the purified C. closterium can be cultured as a biodiesel producer or a nutrition supplement producer.

关 键 词：Cylindrotheca closterium antibiotics sensitivity fatty acid composition total lipid content 

分 类 号：Q949.2[生物学—植物学]