IL-31RA基因敲除小鼠纯合子模型的建立  

Establishment of IL-31RA Gene Knockout Homozygous Mice Model

作　　者：江涛[1] 高婧[1] 岳欢[1] 高雅倩[1] 黄俊琼[1] 

机构地区：[1]遵义医学院附属医院,贵州遵义563000

出　　处：《贵阳医学院学报》2015年第3期229-233,共5页Journal of Guiyang Medical College

基　　金：国家自然科学基金资助项目(No:81260266);贵州省优秀科技教育人才省长专项基金项目(No:黔省专合字200951)

摘　　要：目的:建立IL-31RA基因敲除小鼠纯合子模型,为IL-31RA基因相关研究提供动物模型。方法:IL-31RA基因敲除小鼠严格按照SPF级要求的动物饲养标准进行饲养繁殖,采用聚合酶链式反应(PCR)法鉴定子代小鼠的基因型,RT-PCR法鉴定小鼠IL-31RA mRNA的表达,Western blot鉴定IL-31RA蛋白的表达,HE染色观察小鼠重要脏器的形态学变化。结果:PCR法成功检测出子代小鼠的3种基因型,纯合子基因敲除小鼠未检测出IL-31RA mRNA和IL-31RA蛋白的表达,IL-31RA基因敲除小鼠的重要脏器的形态学特征与野生型小鼠比较无明显变化;基因敲除小鼠可成功饲养繁殖,亦可获得较多的基因敲除纯合子小鼠。结论:成功构建了IL-31RA基因敲除小鼠纯合子模型。Objective： To establish the IL-31 RA gene knockout homozygous mice model and lay the foundation for further study on IL-31 gene. Methods： IL-31 RA gene knockout mice were bred and reproduced according to the SPF class animal feeding standard. PCR was used to identify the genotype of the offspring,the expression of IL-31 RA mRNA was detected by RT-PCR,expression of IL-31 RA protein was detected by Western blot,and morphological changes of vital organs were observed by HE staining. Result： Three genotypes of the offspring of IL-31 RA gene knockout mice were successfully identified; expression of IL-31 RA mRNA and IL-31 RA protein was not detected in IL-31 RA gene knockout homozygous mice. Compared with the wild type mice,morphological characteristics of vital organs of had no significant changes in IL-31 RA gene knockout homozygous mice. IL-31 RA gene knockout mice could be dred and reproduced successfully. Conclusion： The IL-31 RA gene knockout homozygous mice model has been successfully established.

关 键 词：白细胞介素31 基因敲除 聚合酶链反应(PCR) 反转录PCR WESTERN BLOT 模型 动物 

分 类 号：R332[医药卫生—人体生理学]