弓形虫PRU株感染妊娠期小鼠动物模型的建立  

Establishment of Pregnant Mice Infection Model with Prugniaud Strain of Toxoplasma gondii

作　　者：程树强[1] 王正蓉[1] 

机构地区：[1]贵阳医学院附院临检科,贵州贵阳550004

出　　处：《贵阳医学院学报》2015年第3期257-259,263,共4页Journal of Guiyang Medical College

摘　　要：目的:探讨建立弓形虫生殖毒性妊娠期小鼠动物模型的方法。方法:将妊娠期ICR小鼠随机分为孕早期感染组、孕晚期感染组及相应对照组,孕早期和孕晚期感染组小鼠再分别分为3小组,分别以灌胃方式感染弓形虫PRU株包囊10、20及40个,对照组灌胃生理盐水,观察各组小鼠不良妊娠结局及产仔数量。结果:弓形虫PRU株感染数量为10个包囊时,孕早期感染组孕鼠的死亡率为36.36%,出现阴道流血、流产、早产等现象,不良妊娠结局高达85.71%,与对照组比较差异有统计学意义(P<0.05);感染20包囊和40包囊时,孕早期感染组孕鼠的死亡率高达50%和100%;孕晚期感染组孕鼠在妊娠期发病不明显,产仔鼠数量较对照组减少,但差异无统计学意义(P>0.05);结论:用10个PRU株包囊灌胃能建立妊娠期弓形虫感染小鼠动物模型,为研究弓形虫PRU株所致生殖毒性奠定基础。Objective： To establish a suitable pregnant mice infection model with Prugniaud（ PRU）strain of Toxoplasma gondii. Methods： ICR mice were randomly divided into early-pregnancy infection group,late-pregnancy infection group and the control group,and mices of each infection group were divided into 3 groups and were orally infected with 10,20 and 40 PRU strain cysts respectively,control group was given normal saline instead. Adverse pregnancy outcomes and litter amount were observed. Results： When infected with 10 PRU strain cysts,mortality of early-pregnancy mice was36. 6%,and vaginal bleeding,abortion,premature phenomena appeared,the incidence of adverse pregnancy outcomes reached to 85. 71%,there were significant differences compared with control group（ P〈0. 05）. Mortality of early-pregnancy mice was 50% and 100% respectively when infected with 20 cyst and 40 cyst. The morbidity of mice in late-pregnancy infection group was low,and litter amount reduced,but compared with the control group,there was no significant difference（ P〉0. 05）.Conclusions： Pregnant mice infection model is established with 10 PRU strain cysts of Toxoplasma gondii,which lays the foundation for the further study of reproductive toxicity of PRU strain.

关 键 词：弓形虫病 动物 感染 模型 动物 小鼠 近交ICR 妊娠 

分 类 号：R382.33[医药卫生—医学寄生虫学] R531.8[医药卫生—基础医学]