4种两栖爬行动物血细胞的显微结构及细胞化学特征观察  

Observations of Microscopic Structures and Cytochemical Characteristics of Blood Cells in Four Species of Amphibians and Reptiles

作　　者：金星星[1] 张耀光[1] 陈幕飞[1] 金丽[1] 

机构地区：[1]西南大学生命科学学院,淡水鱼类资源与生殖发育教育部重点实验室,水产科学重庆市市级重点实验室,重庆400715

出　　处：《四川动物》2015年第2期223-228,共6页Sichuan Journal of Zoology

摘　　要：光镜下两栖动物中华蟾蜍Bufo gargarizans和牛蛙Rana catesbeiana、爬行动物铜蜓蜥Sphenomorphus indicus和青海沙蜥Phrynocephalus vlangalii4种动物外周血中均可观察到红细胞、血栓细胞、淋巴细胞、单核细胞、嗜中性粒细胞和嗜碱性粒细胞,中华蟾蜍和牛蛙的外周血中还观察到嗜酸性粒细胞。细胞化学染色显示:脂类存在于中华蟾蜍和牛蛙的单核细胞和粒细胞中,2种爬行动物的血细胞中未发现脂类。4种动物的血细胞中均未检测到碱性磷酸酶,但均检测到酸性磷酸酶,在中华蟾蜍和牛蛙血细胞中含量最多。过氧化物酶存在除青海沙蜥以外的3种动物的血细胞中,以在嗜中性粒细胞中居多;4种动物的外周血细胞中均检测到酸性-α-醋酸萘酯酶。To investigate the similarities and differences of blood components in Bufo gargarizans, Rana catesbeiana, Sphenomorphus indicus and Phrynocephalus vlangalii, the microstructure and cytochemistry methods were used in this study. The results showed that the typical six cell types （ erythrocyte, monocyte, lymphocyte, thrombocyte, neutrophil and baso- phil） were observed in peripheral blood cells of the four species, while eosinophil was only observed in the two amphibians （B. gargarizans and R. catesbeiana）. Lipids only existed in monocyte and granular leukocyte of the two amphibians. The results from the color reactions showed that alkaline phosbpatase in all blood cells of the four species was negative, while acid phosphatase widely existed in the peripheral blood cells of the four species. Peroxidase were detected in the peripheral blood cells of the tested species （ except P. vlangalii）, and the majority of peroxidase existed in neutrophil, c^-Naphthyl ac- etate esterase was found in the peripheral blood cells of the four species.

关 键 词：两栖动物 爬行动物 血细胞 显微结构 细胞化学 

分 类 号：Q952[生物学—动物学]