槲皮素纳米结构脂质载体的制备和表征  

Preparation and characterization of quercetin loaded nanostructured lipid carriers

作　　者：倪石磊[1,2] 夏强[1,2,3] 

机构地区：[1]东南大学生物电子国家重点实验室,江苏南京210096 [2]东南大学苏州纳米技术协同创新中心,江苏苏州215123 [3]苏州纳康生物科技有限公司,江苏苏州215123

出　　处：《日用化学品科学》2015年第3期37-42,共6页Detergent & Cosmetics

基　　金：科技部国际科技合作资助项目(2008DFB50060);江苏省科技成果转化资助项目(BA2014096)

摘　　要：采用高压均质法制备QT-NLC并对均质工艺参数进行优化;测定其粒径大小和分布、Zeta电位以及包封率;考察QT-NLC的离心、冻融和静置稳定性;对QT-NLC的抗氧化能力、体外释放和细胞毒性进行评价。实验结果表明,得到的QT-NLC体系的粒径分布均一,包封率为(93.50±0.35)%。体系具有良好的物理稳定性和抗氧化活性。QT-NLC体系具有一定缓释作用,在一定浓度范围内对He La细胞没有毒性。Quercetin loaded nanostructure lipid carriers （ QT - NLC ） and its physico-chemical properties were studied in this paper. QT - NLC was prepared using high pressure homogenization （HPH） method and the process parameters were optimized at the same time. The particle size distribution, Zeta potential and entrapment efficiency （EE） were determined; the long-term physical stability of QT-NLC at centrifugal, freezing and thawing conditions were investigated; the antioxidant capacity, in vitro release and cell toxicity of QT - NLC were evaluated. The results showed that obtained QT - NLC had homogeneous particle size distribution, and the EE was （93.50 ∽ 0.35）%. The system demonstrated a good physical stability and antioxidant activity. The QT- NLC showed sustained-release effect, and had no cytotoxicity to HeLa cells in a certain co ncentration range. In conclusion, the QT - NLC system, with simple preparation method and good physico-chemical properties, can have a great application prospect in medicine, cosmetics and other fields.

关 键 词：槲皮素 纳米结构脂质载体 稳定性 体外释放 细胞毒性 

分 类 号：TQ460.1[化学工程—制药化工]