一种改良ePTFE人工硬脑膜的实验研究  

A Kind of Modified ePTFE Dura Mater: an Experimental Study

作　　者：周峰[1] 方晓东[1] 应广宇[1] 胡未伟[1] 沈宏[1] 

机构地区：[1]浙江大学医学院附属第二医院神经外科,浙江杭州310009

出　　处：《现代生物医学进展》2015年第8期1406-1408,共3页Progress in Modern Biomedicine

基　　金：浙江省科技厅钱江人才项目(2010R10)

摘　　要：目的:对一种e PTFE人工硬脑膜表面改性,将其外表面疏水特性进行改良,并观察其对兔硬脑膜缺损愈合的影响。方法:对一种e PTFE材料人工硬脑膜外表面进行光化学修饰,从而改变其对成纤维细胞吸附能力及促增殖能力。将表面改性后的人工硬脑膜和未改性人工硬脑膜修补兔硬脑膜缺损,观察其对术后1周、3周伤口愈合及脑脊液漏、浸润成纤维细胞、纤维组织厚度等的影响。结果:未处理组2只术后早期出现皮下积液,处理组无术后皮下积液的发生(P=0.12)。人工硬脑膜移植术后1周其外表面成纤维细胞数目表面改性组要明显多于未处理组(P<0.05)。人工硬脑膜移植术后3周其外表面纤维组织厚度表面改性组和未处理组无明显差异(P>0.05)。经过表面改性后的e PTFE人工硬脑膜其促进组织愈合能力要优于未改性组。结论:对e PTFE材料人工硬脑膜进行表面改性处理是一种可行有效的改良方法。Objective： To observe the influence of a kind of modified ePTFE artificial dura mater on the healing process of rabbit dura mater defect. Methods： A kind of ePTFE artificial dura mater was modified for its external surface by photo-activated chemical cross-linking. The surface modified（treatment group）and unmodified （control group） dura mater were transplanted to repair the defected dura mater in a rabbit model. The wound healing, cerebrospinal fluid leakage, infiltrated fibroblast cells and the thickness of fibrous tissue were observed in 1 week and 3 weeks after the transplantation. Results： Subcutaneous effusion occurred in 2 rabbits of control group and none in treatment group in 1 week after transplantation （P =0.12）. The number of fibroblasts in the external surface of artificial dura mater 1 week after transplantation in the treatment group was significantly greater than control group （P〈0.05）. The thickness of fibrous tissues covering artificial dura mater 3 weeks after transplantation in the treatment group was similar to that in control group （P〉0.05）. The healing process of modified dura mater was better than the unmodified dura mater. Conclusion： The photo-activated chemical cross-linking for this ePTFE artificial dura mater is effective to improve the quality of artificial dura mater.

关 键 词：膨体聚四氟乙烯(ePTFE) 人工 硬脑膜 

分 类 号：Q81[生物学—生物工程] Q95-3