Obestatin对血浆ghrelin和nesfatin-1水平及胃排空的影响  

The Effects of Obestatin on Plasmaacyl Gheelin and Nesfatin-1 Levels,and on Gastric Emptying in Rats

作　　者：孙全波[1,2] 栾晓[1] 郭菲菲[1] 孙向荣[1] 祝海 逄明杰 王萍 谷方[1] 徐珞[1] 

机构地区：[1]青岛大学医学院病理生理学教研室,山东青岛266021 [2]菏泽医学专科学校,山东菏泽274031 [3]青岛市立医院,山东青岛266042

出　　处：《现代生物医学进展》2015年第9期1628-1631,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(31071014;81100260;81300281;81470815;81270460);青岛市科技局项目(13-1-4-170-jch;14-2-3-3-nsh)

摘　　要：目的:探讨侧脑室注射obestatin对大鼠血浆酰基化ghrelin、去酰基化ghrelin、nesfatin-1水平的影响以及对胃排空的调控。方法:侧脑室注射obestatin,采用酶免疫测定(EIA)法检测血浆酰基化ghrelin、去酰基化ghrelin、nesfatin-1水平以及胃排空率的变化。结果:侧脑室分别注射0.1、0.3或1.0 nmol obestatin,大鼠血浆酰基化ghrelin、去酰基化ghrelin以及nesfatin-1水平无显著改变(P>0.05),且酰基化ghrelin与去酰基化ghrelin比率无显著改变(P>0.05);侧脑室注射obestatin,大鼠摄食量无显著改变,但胃排空率明显增加(P<0.05);胃排空率明显延迟(P<0.05)。与侧脑室注射1.0 nmol Obestatin组相比,注射1.0 nmol Obestatin+CRF,大鼠摄食量无显著改变,胃排空率明显延迟(P<0.05)。各组摄食量及进入十二指肠内食物量无明显差异(P>0.05)。结论:中枢obestatin促进大鼠的胃排空,可能与h/r CRF通路有关。Objective： To discuss the impact of intracerebroventricular obestatin on plasma acyl ghrelin, des-acyl ghrelin and nesfatin-1 levels, and the regulation of gastric emptying in rats. Methods： Rats were administrated obestatin through intracerebroventricular （ICV） injection; Enzyme immunoassay （EIA） method was used to detect the plasma levels of acyl ghrelin, des-acyl ghrelin, nesfatin-1 and changes in the rate of gastric emptying. Results： After lateral ventricle injection respectively 0.1, 0.3 or 1.0 nmol obestatin, the plasma levels of acyl ghrelin, des-acyl ghrelin, nesfatin-1 had no significant change （P〉0.05）, and the ratio of acyl ghrelin and des-acyl ghrelin had no significant difference （P〉0.05） too. Food intake of rats had no obvious difference after lateral ventricle injection of obestatin, but the gastric emptying rate increased significantl （P〈0.05）. Gastric emptying rate delayed significantly （P〈0.05）. Compared with lateral ventricle injection 1.0 nmol Obestatin group, injection 1.0 nmol Obestatin ＋ CRF, no significant changes in food intake in rats significantly delayed gastric emptying rate （P〈0.05）. While the amount of food intake and the food into the duodenum had no obvious difference （P〉0.05）. Conclusions： Central obestatin promotes gastric emptying of rats, which may be associated with h/rCRF pathways.

关 键 词：胃排空 GHRELIN NESFATIN-1 OBESTATIN 

分 类 号：Q95-3[生物学—动物学] R338[医药卫生—人体生理学]