邻苯二甲酸二(2-乙基己基)酯对大鼠胎盘组织的影响  

Effect of di(2-ethylhexyl) Phthalate Exposure on Rat Placenta

作　　者：顾潇[1,2] 徐婉[2] 王心[2] 尚丽新[2] 余辉[2] 

机构地区：[1]第四军医大学,陕西西安710032 [2]北京军区总医院妇产科,北京100700

出　　处：《现代生物医学进展》2015年第9期1643-1646,共4页Progress in Modern Biomedicine

基　　金：全军"十一五"科技攻关项目(06G019)

摘　　要：目的:探讨孕期不同剂量邻苯二甲酸二(2-乙基己基)酯(DEHP)暴露对大鼠胎盘组织形态学和超微结构的影响,以推断DEHP对胎盘结构和功能的损害。方法:健康雌性Wistar大鼠40只,随机分为4组,妊娠第11日起每日给予DEHP灌胃,剂量分别为:对照组(玉米油)、低剂量组(100 mg/kg)、中剂量组(500 mg/kg)和高剂量组(1000 mg/kg),妊娠第19日处死孕鼠,取胎盘组织分别做HE染色和电镜制片,观察各剂量组胎盘形态学及超微结构的变化。结果:各剂量组胎盘形态学和超微结构的变化与DEHP摄入量呈负相关,低剂量组胎盘无明显改变;中剂量组胎盘缩小,空泡化细胞增多,微观结构显示胞质内线粒体水肿;高剂量组胎盘迷路带血窦扩张淤血严重,滋养细胞变性、坏死。结论:DEHP可导致大鼠胎盘形态结构发生改变,这种病理改变是胎盘功能减退的形态学基础,可直接影响胚胎发育和妊娠结局。Objective： Discuss the effect of different doses of DEHP exposure on placental tissue morphology and ultra-structure changes in pregant rats, and conclude DEHP have adverse impact on the structure and function of placental. Methods： 40 healthy female Wistar rats were randomly divided into 4 groups, that is control group （corn oil）, low dose group （DEHP 100 mg/kg）, middle dose group （DEHP 500 mg/kg） and high dose group （DEHP 1000 mg/kg）. On eleventh day of pregnancy, rats were given DEHP/corn oildaily respectively by intragastric administration.On the nineteenth day pregnant rats were killed, placental tissues were studied with HE staining and electron microscope, to observe the morphology and ultra-structure changes of each dose group. Results： Placental tissue morphology and ultra-structure changes were negatively correlated with DEHP daily intake. Low dose group had no obvious change; the placental in middle dose group were shrinked, vacuolated cells increased, mitochondria edema in the cytoplasm was detected by observing the microstructure. Labyrinth blood sinus in high dose group of placental were dilated and congestion seriously, trophoblastic cells were degeneration and necrosis. Conclusions： DEHP can cause the changes of morphological structure in rat＇s placental, the pathological change is the morphological basis of placental dysfunction, it can directly affect the development of embryos and pregnancy outcome.

关 键 词：邻苯二甲酸二(2-乙基己基)酯(DEHP) 孕鼠 胎盘 

分 类 号：Q95-3[生物学—动物学] R714[医药卫生—妇产科学]