成骨细胞介电特性检测的方法研究  

Research on the Method of Detecting Dielectric Properties of Osteoblast

作　　者：刘玺[1,2] 丁冲[1,2] 王哲[1,2] 张健[1,2] 商澎[1,2] 

机构地区：[1]西北工业大学生命学院 [2]西北工业大学特殊环境生物物理学研究所 空间生物实验模拟技术国防重点学科实验室,陕西西安710072

出　　处：《现代生物医学进展》2015年第9期1764-1768,1771,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(51147008)

摘　　要：目的:以小鼠成骨细胞为材料,建立一套完整的用于成骨细胞的介电特性检测方法。方法:本研究首先是设计制作不同形状和尺寸的细胞介电谱检测装置(测量池),其次进行测量池的生物相容性评价,包括浸提液毒性实验,HE染色观察细胞形态。最后将细胞培养在测量池中,连入阻抗分析仪在20Hz^10MHz检测并联电导Gp和电容Cp并绘制介电谱图。结果:生物相容性检测表明测量池对细胞无毒性,可使用。此外,介电特性检测结果表明随着频率的增大,细胞的电容减小,电导略微增大后减小;随着培养时间的增长,电容值增大而电导值减小;经中强磁场处理后,细胞的电容和电导值都有下降。结论:该方法能够为其他贴壁细胞的介电特性检测提供研究基础,并对于在此基础上展开的贴壁细胞介电特性解析提供参考。Objective： To establish a complete set of dielectric properties detective method for osteoblast by using mouse os- teoblast. Methods： Different shape and size of cell dielectric spectroscopy detection device （dielectric cell） was designed and made, and the biocompatibility, including the toxicity test of leach liquor, HE staining was measured to observe the cell morphology. The cell were cultured in the measuring vessel, parallel conductance Gp and parallel capacitance Cp in 20Hz-10MHz was detected by impedance ana- lyzer and draw the dielectric spectra. Results： Biocompatibility testing showed that the dielectric cell had no toxicity to cell and it can be used. Dielectric properties detection results showed that with the increase of frequency, the capacitance decreased, conductance slightly increased and then decreased. With the growth of the culture time, the Cp and Gp values all decreased. Moderate intensity magnetic field treated showed that the Cp and Gp values all declined. Conclusions： This method can provide a research bases for other dielectric proper- ty detective of adherent cells, and it can also provide a reference for adherent cells analysis based on this method.

关 键 词：介电特性 介电检测 介电谱 测量池 

分 类 号：Q61[生物学—生物物理学] Q813