棉籽糖低钾右旋糖酐液中灌注丹酚酸B对大鼠离体肺的保护作用  

Protective Effect and Mechanism of Raffinose-low Potassium Dextran Added Salvianolic Acid B on Isolated Lung in Rats

作　　者：孙敏[1] 李慧星[1] 徐辉[1] 陈静瑜[1] 孙洁[1] 纪勇[1] 

机构地区：[1]南京医科大学附属无锡市人民医院胸外科,江苏无锡214023

出　　处：《现代生物医学进展》2015年第11期2048-2052,共5页Progress in Modern Biomedicine

摘　　要：目的:研究丹酚酸B对离体供肺的保护作用。方法:将SD大鼠36只,随机分为对照组、低浓度实验组和高浓度实验组,每组各12只。对照组选择棉籽糖低钾右旋糖酐液(R-LPD液),实验组在对照组灌注液的基础上分别加入丹酚酸B注射液800mg/L、1200 mg/L。呼吸机辅助通气下边灌注边取下供肺,离体后再用灌注液行顺灌及逆灌,后放入盛有4℃相应保存液的无菌冰桶内保存。术后6 h、9 h、12 h、24 h分别测两组供肺的湿干重比(W/D)、髓过氧化物酶(MPO)活性、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性。结果:各组内12 h前相邻时间点比较,各参数均无统计学差异(P>0.05);24 h后各参数明显高于12 h(P<0.01)。各组间同一时间点比较,12 h前组间各参数无统计学差异(P>0,05);保存至24 h,W/D、MDA含量、MPO活性比较,对照组>低浓度实验组>高浓度实验组(P<0.05);而SOD活性比较,对照组<低浓度实验组<高浓度实验组(P<0.05)。光镜下各肺组织保存至12 h以前,病理形态学均无明显炎症损伤,仅表现轻度炎症反应。12 h后对照组组光镜病理观察出现肺泡结构破坏、间质水肿、炎症损伤较低浓度实验组组明显,高浓度实验组情况较低浓度实验组肺组织结构更为清晰且炎症损伤较轻。结论:加入丹酚酸B后的灌注液在12 h前未有明显优势,12 h后能更加有效地保证小鼠供肺质量,使供体肺在离体情况下有效保存更长的时间,且高浓度丹酚酸B能更有效的提高对供体肺的保护发挥了重要作用。Objective： To research the protective effect of Salvianolic acid B on isolated lung in rats. Methods： 36 SD rats were randomly divided into 3 groups of control group, low-dose experimental group and high-dose experimental group with 12 rats each.Isolated lung in control group was perfused with raffinose-low potassium dextran（R-LPD）.The experimental group which was added 800mg/L or 1200 mg/L Salvianolic acid B subdivided into low or high dose experimental group. Meanwhile, we set up the model of isolated lung in all these rats. Wet/dry weight ratio（W/D）, myeloperoxidase（MPO）, malondialdehyde（MDA） and superoxide dismutase（SOD）were measured and pulmonary structure was observed by HE staining at different preservative periods of 6 h, 9 h, 12 h and 24 h after infusion. Results： Intragroup comparison was made in these groups： all parameters had no statistical difference before 12 h. However,W/D、MPO、MDA、SOD in 24 h were higher than those in 12 h（P0.01）. After 6, 9 and 12-hour preservation, all parameters of these groups had no significant difference. However, W/D, MDA and MPO were lower after 24-hour preservation in experimental group, and SOD was higher in experimental group（P0.05）, and it was detected more obviously in high-dose experimental group. Moreover, before12-hour preservation, these three groups did not show inflammatory injury, only manifested slight inflammatory reaction through histologic observation. Destructive pulmonary structure, alveolar interstitial edema and inflammatory damage were more clearly in control group than in experimental group. Conclusion： The perfusion that contained with Salvianolic acid B had no apparent superiority before 12-hour. However, after 12-hour, it could ensure the quality and prolong the preservation time of the donor lungs more effectively,and high dose of Salvianolic acid B might play an important role in donor lungs protection.

关 键 词：丹酚酸B 供体保存 抗氧化 肺移植 

分 类 号：Q95-33[生物学—动物学] R655.3[医药卫生—外科学]