胶体金免疫层析技术的研究进展  

Progress of the Colloidal Gold-based Immunochromatographic Assay

作　　者：张莹[1] 冯海 吴秋平[1] 谈增 贺加贝[2] 

机构地区：[1]浙江工业大学药学院,浙江杭州310014 [2]天津大学药物科学与技术学院,天津300072

出　　处：《现代生物医学进展》2015年第12期2349-2351,共3页Progress in Modern Biomedicine

摘　　要：胶体金免疫层析技术是一种常用的免疫层析检测方法,由于其操作简单、省时、制造成本较低、结果易判读等特点,非常适合于现场检测,广泛用于生物、医药、食品等领域。但该技术也存在一些不足,如质量控制困难、灵敏度低、不易定量等,在一定程度上限制了其应用。本文,简述了该技术的原理、特点和国内外研究进展,重点综述了该方法的不足及改进的可能性,为拓宽其应用范围提供帮助。Colloidal gold-based immunochromatographic assay is a common used lateral flow immunoassay, which offers the advantage of simplicity, rapidity, relatively low manufacturing costs and easy evaluation, and it is ideally suited for on site testing, and widely used in biology, medicine, food and other fields. But there are some disadvantages, such as, low sensitivity, being difficult to quality control hard to quantitative detection and so on. The shortcomings limit its application in a certain extent. This paper describes the principle, characteristics and domestic and foreign progress of the method, and emphasizes on its lacks and the possibilities for improvement, so as to help to broaden its array of new application areas.

关 键 词：胶体金 免疫层析 灵敏度 定量检测 多项检测 

分 类 号：R392.33[医药卫生—免疫学]