集卵薄涂片法检测牛粪中血吸虫卵含量的研究  

STUDIES ON ASSESSMENT OF INTENSITY OF INFECTION FOR SCHISTOSOMA JAPONICVM IN CATTLE

作　　者：姚邦源 钱珂[1] 祝红庆[1] 朱丹[1] 陈韶红 张容[1] 

机构地区：[1]中国预防医学科学院寄生虫病研究所

出　　处：《中国寄生虫学与寄生虫病杂志》1991年第4期293-297,共5页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：世界卫生组织西太区办事处部分财政资助

摘　　要：本文比较了薄涂片法和透明法的虫卵回收率。结果表明,在三种虫卵梯度,薄涂片法的虫卵回收率,分别为透明法的18、4.2和3.5倍,前者的变异系数明显低于后者;现场检查34头孵化阳性黄牛的结果也证实薄涂片法优于透明法。薄涂片法检查半量粪渣(制6张涂片)的虫卵估计值和标准差与全量粪渣结果相似,可以代替全量粪渣检查。在感染度较高的地区,可以抽样检查1/4粪渣,制3张涂片检查,从而减少工作量。In order to carry out quantitative epidemiological survey of animal schistosomiasis, a sensitive egg-counting method was studied both in the laboratory and in the field.Fecal material was filtered by a copper gauze (60 meshes/inch) into a nylon-tissue bag with 2 layers, 150 and 260 meshes/inch respectively. Thin smears were made from the residue in the bag.The mean recovery rate of mature schistosome eggs by previously adding 100 eggs to 5g of feces from noninfected cattle was 67.5%, ranging from 53 to 81%.To compare the egg-concentration thin smear method mentioned above,with egg-concentration thick smear method after hyalinization, various numbers of eggs, namely, 25, 50 and 100, were added to 5g of feces. The mean recovery rates of fecal eggs were 57.6%, 57.6% and 69.1% respectively, for the thin smear method and 3.2%, 13.8% and 19.5% for the thick smear method. The coefficients of variability in the former were from 12.9% to 17.4% and in the latter, from 30.7% to 98.8%.The total number of schistosome eggs per 5g of feces found in 34 egg-positive cattle in the field were 434 and 178 by the thin smear method and the thick smear method, respectively.A correlation analysis performed on the numbers of detected eggs in a total volume of fecal sediment and from half a volume of fecal sediment multiplied by two, showed a correlation coeffecient of 0.98 (P<0.01), indicating that half a volume of fecal sediment may be used instead of a whole volume. The value of EPG, X, can be calculated from 5g of feces by applying the following formula:X(EPG)=total number of eggs in 6 slides × 2/5The adjustment value to estimate the true value of EPG from the detected one is Y= 1.1 + 1.4X.The results indicated that the egg-concentration thin smear method was significently better than the thick smear method in terms of sensitivity and reliability, so the former was considered to be useful in field epidemiological survey for animal schistosomiasis.

关 键 词：血吸虫 感染度 虫卵回收率 

分 类 号：R532.210.4[医药卫生—内科学]