中国兰叶色突变体生理生化分析  

Research on Physiological and Biochemical Characters of Leaf Color Mutants in Chinese Ochid

作　　者：蒋彧[1] 何俊蓉[1] 熊剑锐[1,2] 李萍[2] 卓碧萍[1] 

机构地区：[1]四川省农业科学院园艺所,农业部西南地区园艺作物生物学与种质创制重点实验室,四川成都610066 [2]西南交通大学生命科学与工程学院,四川成都610031

出　　处：《北方园艺》2015年第7期65-68,共4页Northern Horticulture

基　　金：四川省应用基础研究计划资助项目(2014JY0106);公益性行业(农业)科研专项经费资助项目(201203071)

摘　　要：以中国兰春剑‘隆昌素’和其叶色突变体‘叶艺隆昌素’为试材,通过测定组培苗叶片及根状茎增殖重量、分化率及生理生化指标,研究了生理生化特性对叶色突变体增殖与分化效率的影响。结果表明:叶艺突变体根状茎增殖、分化能力及分化苗平均株高均降低。叶色突变体试管苗叶绿素a、叶绿素b、叶绿素a+b含量分别为对照的16.71%、15.86%、16.49%,且在叶色突变体根状茎分化成苗的过程中,叶绿素b含量增加,而叶绿素a含量降低,而类胡萝卜素相对含量高。可溶性糖含量、可溶性蛋白质含量降低,丙二醛含量及相对电导率升高;活性氧清除酶系中过氧化物酶活性升高,超氧化物歧化酶活性和过氧化氢酶活性降低。可见叶色突变体的增殖与分化效率与其生理生化特性密切相关。The in vitro shoots of Cymbidium longibracteaturn‘Longchangsu＇with verge line pattern leaves and normal Cymbidium longibracteaturn‘Longchangsu＇as materials,the effect of multiplication and differentiation on the mutants were studied by the detection of the multiplication weight and differentiation rate and physiological and biochemical index determination of the leaves and rhizomes.The results showed that the rhizomes＇ multiplication and differentiation were lower than those of the contrasts,so was the average height.The mutants＇ chlorophyll a and chlorophyll b and chlorophyll a＋b were 16.71%,15.86%,16.49%of the contrasts respectively.Chlorophyll b and carotenoids/chlorophyll were increased while chlorophyll a was decreased during the differentiation from the rhizomes to shoots.The contents of soluble sugar and protein were decreased.MDA content and the relative conductivity were increased.The activities of antioxidant enzymes,such as catalase（CAT）and superoxide dismutase（SOD）were lower while peroxidase（POD）activity were higher in the mutants than in the contrasts.The multiplication and differentiation of the mutants were closely related to the physiological characteristics.

关 键 词：中国兰 叶色突变体 生理生化特性 

分 类 号：S682.31[农业科学—观赏园艺]