新宁云锦杜鹃组织培养研究  

Study on Tissue Culture of Rhododendron fortunei in Xinning

作　　者：唐映红[1,2] 沈帆[1] 刘芳[1] 郭清泉[1] 康用权 陈建荣[1] 

机构地区：[1]长沙学院生物与环境工程系,湖南长沙410022 [2]湖南农业大学农学院,湖南长沙410128 [3]湖南湘植园林科技有限公司,湖南长沙410116

出　　处：《北方园艺》2015年第7期94-97,共4页Northern Horticulture

基　　金：长沙市科技计划资助项目(K1205040-31);湖南省科技计划资助项目(2014CK4018);长沙学院人才引进资助项目(SF080402)

摘　　要：以新宁云锦杜鹃茎段为外植体,利用植物组织培养技术,采用不同的外植体消毒方案,以1/4MS为基本培养基,添加了不同浓度的6-BA、ZT和NAA等植物生长调节物质,研究了不同培养条件对云锦杜鹃离体芽诱导、增殖、生根的影响。结果表明:最佳外植体消毒方案为75%乙醇30s+5%次氯酸钠10min,转瓶6次去除内生菌,芽诱导的最佳培养基配方是1/4MS+7mg/L ZT+0.15mg/L NAA,离体芽在1/4MS+1.0mg/L ZT+0.10mg/L NAA培养基上的增殖效果较好,幼苗在1/2MS+0.1mg/L 6-BA+1.5mg/L NAA培养基上的生根效果较好。Taking leaves and stems of Rhododendron fortunei as explants,using plant tissue culture techniques,different explant disinfection programs were conducted and using 1/4MS as the basic medium supplemented with different concentrations of ZT and NAA plant growth regulating substances,in order to study the effect of different culture conditions on Rhododendron in vitro bud induction and proliferation.The results showed that the best explant disinfection program was 75%ethanol for 30 seconds and 5%sodium hypochlorite for 10 minutes,and the shoot apex had the highest induced rate.The best bud induction medium program was 1/4MS mixed with 7mg/L ZT and 0.15mg/L NAA,in vitro shoots had the best proliferation effect in medium with 1/4MS mixed with 1.0mg/L ZT and 0.10mg/L NAA,seedling had the best rooting effect in medium with 1/2MS＋0.01mg/L 6-BA＋1.5mg/L NAA.

关 键 词：云锦杜鹃 组织培养 培养基 

分 类 号：S685.210.36[农业科学—观赏园艺]