AB-8大孔吸附树脂静态纯化北五味子木脂素工艺研究  

Study on Technology of Static Purification of Lignans fromSchisandra chinensis Baill by Macroporous Adsorption Resin

作　　者：程振玉[1] 宋海燕[2] 杨英杰[1] 薛俊礼 吉惠杰[1] 

机构地区：[1]吉林化工学院化学与制药工程学院,吉林吉林132022 [2]吉林农业科技学院实验中心,吉林吉林132101

出　　处：《北方园艺》2015年第7期141-145,共5页Northern Horticulture

摘　　要：以五味子醇甲、五味子酯甲、五味子甲素、五味子乙素和五味子丙素为研究指标,以高效液相色谱作为检测方法,在考察不同类型大孔吸附树脂吸附和解析性能的基础上,对最佳树脂静态纯化北五味子木脂素的工艺进行探索。结果表明:AB-8大孔吸附树脂纯化效果最佳,最佳吸附条件为:提取液pH值为6.6,5种木脂素总浓度为161.815mg/L,1g大孔吸附树脂加入150mL提取液,于55℃振荡吸附10h;最佳解析条件为:以无水乙醇为解析剂,在65℃下解析8h;在此条件下,5种木脂素的总含量由粗体物浸膏中的0.98%上升到5.86%。该方法为工业化大量生产北五味子木脂素提供了理论依据和数据支撑。Taking Schisandrol A,Schisantherin A,Deoxyschisandrin,Schisandrin B and Schisandrin C as test index,the static purification conditions of lignans were determined on the basis of investigating the adsorption and desorption performance of different kind of macroporous resins.The results showed that,AB-8macroporous resins was chose and employed in this study,the optimal adsorption conditions were as follows：adsorption sample volumes of 40 mL,pH value of 6.6,adsorption temperature of 55℃and adsorption time of 10hours;the optimal desorption conditions were desorption time of 8hours,desorption temperature of 65℃,dehydrated ethanol as desorption solvent and the volume of 150 mL.Under these optimal conditions,the lignans content of Schisandracrude extracts increased from 0.98%to 5.86%.The method proposed in this study provided data and theoretical support.

关 键 词：高效液相色谱法 北五味子 木脂素 大孔吸附树脂 纯化 

分 类 号：R285[医药卫生—中药学]