甲基磺酸乙酯对小新月菱形藻的生物学效应  

The biological effect of the Ethyl Methanesulphonate on Nitzschia closterium

作　　者：杨茂纯 赵耕毛[1] 王长海[1] 

机构地区：[1]南京农业大学资源与环境科学学院,江苏省海洋生物学重点实验室,江苏南京210095

出　　处：《海洋科学》2015年第1期8-12,共5页Marine Sciences

基　　金：国家863计划项目(2012AA021706)

摘　　要：为提高小新月菱形藻(Nitzschia closterium)的产油脂能力、获得油脂含量高的突变株,利用甲基磺酸乙酯(EMS)对该藻株进行了化学诱变处理,并对其生物学作用进行了考察。结果显示,EMS对小新月菱形藻具有较好的诱变作用,经0.1 mol/L和0.05 mol/L EMS处理后分别得到油脂生产能力较强的突变藻株YA和YB,在同等培养条件下两株突变藻单位体积细胞的内叶绿素a、类胡萝卜素、蛋白质、可溶性糖和油脂相对含量分别比对照组提高了30.05%和25.77%、22.15%和19.26%、9.06%和8.27%、48.25%和45.48%、44.06%和12.86%。In order to obtain the high-lipid producing strains of Nitzschia closterium, the method of chemical muta-tion with Ethyl Methanesulphonate （EMS） was used, and the biological effects of EMS on the cells of N. closterium were studied. The results showed that the EMS has good mutagenesis effect on the cells of N. closterium, and two different mutant strains of N. closterium YA and YB were obtained after treatment with 0.1 mol/L and 0.05 mol/L dos-ages of EMS, respectively, which both had stronger oil production capacity. Under the same cultivation condition, the chlorophyll a, carotenoids, protein, soluble sugar contents and lipid relative contents of YA and YB have in-creased by 30.05% and 25.77%, 22.15% and 19.26%, 9.06% and 8.27%, 48.25% and 45.48%, and 44.06% and 12.86%compared with the CK, respectively.

关 键 词：微藻 小新月菱形藻(Nitzschia closterium) 诱变 甲基磺酸乙酯 油脂 

分 类 号：S963[农业科学—水产养殖]