甲苯咪唑与曙红Y相互作用的共振瑞利散射、倍频散射和荧光光谱及其分析应用  

Resonance Rayleigh Scattering,Frequency Doubling Scattering and Fluorescence Spectra of the Eosin Y-Mebendazole System and Their Analytical Applications

作　　者：李勤 田丰玲 周尚[3] 傅凌莉 刘蕖 杨季冬[4] 

机构地区：[1]重庆市医药卫生学校,重庆408000 [2]西南大学化学化工学院,重庆400715 [3]长江师范学院化学化工学院,重庆408000 [4]重庆三峡学院化学与环境工程学院,重庆404100

出　　处：《应用化学》2015年第4期481-488,共8页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(21175015)资助项目;重庆市涪陵区科委资助项目(FLKJ;2013ABB2088)~~

摘　　要：在pH值3.4-3.9的Britton-Robinson（BR）缓冲介质中,甲苯咪唑（MBZ）与曙红Y（EY）反应形成1∶1的离子缔合物,体系反应不仅导致荧光光谱的猝灭,还使共振瑞利散射（RRS）和倍频散射（FDS）显著增强,最大的RRS峰位于326 nm处。荧光猝灭法、RRS法、FDS法的检出限分别为32.31、7.24和11.65μg/L,其中RRS法的灵敏度最高。实验讨论了反应的最佳条件以及共存物质的影响。该方法用于甲苯咪唑片剂以及尿样中MBZ的测定,结果令人满意。In pH 3.4 - 3.9 Britton-Robinson（BR） buffer solution, mebendazole（MBZ） reacts with eosin Y （EY） to form a 1：1 ion-association complex, which not only results in the quenching of fluorescence, but also results in the great enhancement of resonance Rayleigh scattering （RRS） and frequency doubling scattering （FDS）. Furthermore, a new RRS spectrum appears, and the maximum RRS wavelength is located at 326 nm. The detection limit for MBZ is 32. 31 μg/L using the fluorophotometry, 7.24 μg/L by the RRS method and 11.65 μg/L by the FDS method. Among them, the RRS method has the highest sensitivity. The experimental conditions were optimized and effects of coexisting substances were evaluated. These methods have been applied to the determination of MBZ in tablets and human urine samples. The composition and structure of the ion-association complex and the reaction mechanism are discussed.

关 键 词：共振瑞利散射 倍频散射 荧光猝灭 甲苯咪唑 曙红Y 

分 类 号：O657[理学—分析化学]